Recombinant Human CASP9 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: CASP9-3270H
• Product Overview: CASP9 MS Standard C13 and N15-labeled recombinant protein (NP_127463) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 30 kDa
• AA Sequence: MDEADRRLLRRCRLRLVEELQVDQL WDVLLSRELFRPHMIEDIQRAGSGS RRDQARQLIIDLETRGSQALPLFIS CLEDTGQDMLASFLRTNRQAAKLSK PTLENLTPVVLRPEIRKPEVLRPET PRPVDIGSGGFGDVEQKDHGFEVAS TSPEDESPGSNPEPDATPFQEGLRT FDQLDAISSLPTPSDIFVSYSTFPG FVSWRDPKSGSWYVETLDDIFEQWA HSEDLQSLLLRVANAVSVKGIYKQM PGCFNFLRKKLFFKTSTRTRPLEQK LISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: CASP9 caspase 9 [ Homo sapiens (human) ]
• Official Symbol: CASP9
• Synonyms: CASP9; caspase 9, apoptosis-related cysteine peptidase; caspase 9, apoptosis related cysteine protease; caspase-9; APAF 3; ICE LAP6; MCH6; PPP1R56; protein phosphatase 1; regulatory subunit 56; apoptotic protease MCH-6; ICE-like apoptotic protease 6; apoptotic protease activating factor 3; protein phosphatase 1, regulatory subunit 56; APAF3; APAF-3; ICE-LAP6; CASPASE-9c
• Gene ID: 842
• mRNA Refseq: NM_032996
• Protein Refseq: NP_127463
• MIM: 602234
• UniProt ID: P55211

Reviews

04/12/2023

Its purity and consistency have provided me with reliable and reproducible results, ensuring the accuracy of my research findings.

04/27/2020

Their expertise and guidance have helped overcome hurdles, ensuring the smooth progress of my research.

10/27/2016

The bands of proteins in Western Blotting experiments were very clear.

Q&As

What clinical conditions are associated with dysregulation of CASP9 protein?

Dysregulation of CASP9 protein is associated with cancer, neurodegenerative diseases, and immune system disorders.

Can CASP9 protein be used as a therapeutic target in autoimmune diseases?

CASP9 protein is being investigated as a potential therapeutic target in autoimmune diseases to modulate immune cell apoptosis.

How is CASP9 protein implicated in immune system disorders?

Dysregulation of CASP9 protein can lead to abnormal immune cell apoptosis, contributing to autoimmune diseases and immunodeficiency.

How is CASP9 protein targeted in cancer therapy?

CASP9 protein is targeted using small molecule inhibitors and therapeutic antibodies to induce apoptosis in cancer cells.

Are there any genetic variations in the CASP9 gene that affect protein function?

Yes, genetic variations in the CASP9 gene can impact protein function and have been linked to disease susceptibility.