| Description : |
Granulocyte chemotactic protein 2 (GCP-2) also known as Chemokine (C-X-C motif) ligand 6 (CXCL6) is a small cytokine belonging to the CXC chemokine family. As its former name suggests, GCP-2 is a chemoattractant for neutrophilic granulocytes. Among human CXC chemokines, GCP2 is most closely related to ENA78 (78% amino acid (aa) sequence identity in the mature peptide region and 86% identity in the signal sequence). The structure and sequence of the genes for human GCP2 and ENA78 also exhibit close similarity suggesting the two genes may have originated from a gene duplication. GCP2 can signal through the CXCR1 and CXCR2 receptors. |
| Source : |
CHO |
| Species : |
Human |
| Form : |
Lyophilized after extensive dialysis against PBS. |
| Bio-activity : |
The EC50 value of human GCP-2/CXCL6 on Ca2+ mobilization assay in CHO-K1/ Gα15/hCXCR2 cells (human Gα15 and human CXCR2 stably expressed in CHO-K1 cells) is less than 0.8 μg/ml. |
| Molecular Mass : |
~9 kDa |
| Endotoxin : |
< 0.2 EU/μg of protein by gel clotting method. |
| Purity : |
> 98% |
| Storage : |
Upon receiving, this product remains stable for up to 6 months at lower than -70 centigrade. Upon reconstitution, the product should be stable for up to 1 week at 4 centigrade or up to 3 months at -20 centigrade. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml. |
