Cxcl10

Case Study 1: Tadaatsu Imaizumi, 2021

Various viruses including a novel coronavirus (SARS-CoV-2) can infect the kidney. When viruses invade the glomeruli from the bloodstream, glomerular endothelial cells (GECs) initiate the innate immune reactions. Role of IFIT1/2/3 in the expression of C-X-C motif chemokine ligand 10 (CXCL10) was also examined. Human GECs were cultured and stimulated with polyinosinic-polycytidylic acid (poly IC), a synthetic TLR3 agonist. Real-time qPCR, Western blotting, and ELISA were used to examine the expression of IFIT1/2/3, IFN-β, and CXCL10. RNA interference against IFN-β or IFIT1/2/3 was also performed. xpression of IFIT1/2/3 and CXCL10 was induced by poly IC in GECs. Knockdown of IFIT1/2/3 decreased the CXCL10 expression. Knockdown of IFIT3 decreased the expression of IFIT1 and IFIT2 proteins.

Fig1. CXCL10 concentration in the medium was measured using an ELISA kit.

Fig2. The expression of mRNA and protein for IFIT1, IFIT2, IFIT3, and CXCL10 was analyzed using qRT-PCR.

Case Study 2: Michelle Silasi, 2020

The process of implantation, trophoblast invasion and placentation demand continuous adaptation and modifications between the trophoblast (embryonic) and the decidua (maternal). Human chorionic gonadotropin (hCG) is one of the earliest hormones produced by the blastocyst and has potent immune modulatory effects, especially in relation to T cells. This study hypothesized that trophoblast-derived hCG modulates the immune population present at the maternal fetal interface by modifying the cytokine profile produced by the stromal/decidual cells. Using in vitro models from decidual samples the researchers demonstrate that hCG inhibits CXCL10 expression by inducing H3K27me3 histone methylation, which binds to Region 4 of the CXCL10 promoter, thereby suppressing its expression. hCG-induced histone methylation is mediated through EZH2, a functional member of the PRC2 complex. Regulation of CXCL10 expression has a major impact on the capacity of endometrial stromal cells to recruit CD8 cells. Alterations in this immune regulatory function, such as during infection, will have detrimental effects on the success of the pregnancy.

Fig3. Quantification of CXCL10 protein expression in HESC treated with cAMP or decidualized with estrogen, progesterone and cAMP.

Fig4. Human term decidua tissues were treated with LPS (100 ng/ml) for 24 followed by evaluation of CXCL10 protein expression by Luminex.

Cxcl10 involved in several pathways and played different roles in them. We selected most pathways Cxcl10 participated on our site, such as Cytokine-cytokine receptor interaction, Chemokine signaling pathway, Toll-like receptor signaling pathway, which may be useful for your reference. Also, other proteins which involved in the same pathway with Cxcl10 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Cxcl10 has several biochemical functions, for example, CXCR3 chemokine receptor binding, cAMP-dependent protein kinase regulator activity, chemokine activity. Some of the functions are cooperated with other proteins, some of the functions could acted by Cxcl10 itself. We selected most functions Cxcl10 had, and list some proteins which have the same functions with Cxcl10. You can find most of the proteins on our site.

Cxcl10 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with Cxcl10 here. Most of them are supplied by our site. Hope this information will be useful for your research of Cxcl10.

DPP4; DPP8

Gene Family

Research Area

Related articles