Active Recombinant Cynomolgus/Rhesus macaque TNFSF4 protein, mFc-tagged

• Cat.No.: TNFSF4-345R
• Product Overview: Recombinant Cynomolgus/Rhesus macaque TNFSF4 protein(Gln 51 - Leu 183), fused with mFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Cynomolgus/Rhesus macaque
• Tag: mFc
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
• Bio-activity: Immobilized Rhesus macaque OX40, His Tag at 2 μg/mL (100 μL/well) can bind Rhesus macaque OX40 Ligand, Mouse IgG2a Fc Tag with a linear range of 0.1-3 ng/mL.
• Molecular Mass: This protein carries a mouse IgG2a Fc tag at the N-terminus.The protein has a calculated MW of 42.5 kDa. The protein migrates as 47-58 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein Length: Gln 51 - Leu 183
• Endotoxin: Less than 0.1 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.