Active Recombinant Full Length Human DUSP3 Protein, N-GST-tagged
Cat.No. : | DUSP3-184HFL |
Product Overview : | N-terminal GSTtagged, recombinant, human VHR, full length, expressed in E. coli cells. Purified using glutathione agarose. |
- Specification
- Gene Information
- Related Products
Description : | VHR protein is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotryosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. |
Source : | E. coli |
Species : | Human |
Tag : | GST |
Protein length : | Full Length |
Form : | 2.33mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
Bio-activity : | 506U/mg, where one unit of VHR, active activity is defined as the release of 1nmol of phosphate per minute from the phosphorylated substrate 6,8-difluoro-4- methylumbelliferyl phosphate (DiFMUP) at room temperature. |
Molecular Mass : | 47.8 kDa |
AA Sequence : | 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSPGIS GGGGGILMSG 241 SFELSVQDLN DLLSDGSGCY SLPSQPCNEV TPRIYVGNAS VAQDIPKLQK LGITHVLNAA 301 EGRSFMHVNT NANFYKDSGI TYLGIKANDT QEFNLSAYFE RAADFIDQAL AQKNGRVLVH 361 CREGYSRSPT LVIAYLMMRQ KMDVKSALSI VRQNREIGPN DGFLAQLCQL NDRLAKEGKL 421 KP |
Purity : | Purity 98% by SDS-PAGE and Coomassie blue staining. |
Applications : | Drug Discovery & Development |
Storage : | 1 year at -70 centigrade. |
Handling Advice : | Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
Use/Stability : | On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
Gene Name : | DUSP3 dual specificity phosphatase 3 [ Homo sapiens (human) ] |
Official Symbol : | DUSP3 |
Synonyms : | DUSP3; dual specificity phosphatase 3; VHR |
Gene ID : | 1845 |
mRNA Refseq : | NM_004090.4 |
Protein Refseq : | NP_004081.1 |
MIM : | 600183 |
UniProt ID : | P51452 |
Products Types
◆ Recombinant Protein | ||
DUSP3-2939H | Recombinant Human DUSP3 Protein, GST-tagged | +Inquiry |
DUSP3-2781H | Recombinant Human DUSP3 Protein, MYC/DDK-tagged | +Inquiry |
Dusp3-2689M | Recombinant Mouse Dusp3 Protein, Myc/DDK-tagged | +Inquiry |
DUSP3-3217H | Active Recombinant Human DUSP3 protein(Met1-Pro185) | +Inquiry |
DUSP3-1180R | Recombinant Rhesus Macaque DUSP3 Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
DUSP3-001HCL | Recombinant Human DUSP3 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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