Active Recombinant Human AKT3(S472D)(117-end) Mutation Protein, N-His-tagged

• Cat.No.: AKT3-17H
• Product Overview: N-terminal 6Histagged, recombinant human PKB gamma, amino acids 117–end containing S472D mutation. Expressed in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: AKT (also called protein kinase B) is a serine/threonine protein kinase that has been implicated in mediating a variety of biological responses including inhibiting apoptosis and stimulating cellular growth. Three mammalian isoforms (AKT1, AKT2, and AKT3) of the kinase have been identified. EMBL AF124141. The recombinant protein contains an amino acid substitution, S472D, with reference to EMBL AF124141. This mutation mimics phosphorylation of S472.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 117-end
• Form: 3.91mg/ml of enzyme in 50mM Tris/HCl, pH7.5, 150mM NaCl, 0.03% Brij-35, 0.1mM EGTA, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 12357U/mg, where one unit of PKB gamma, active activity is defined as 1nmol phosphate incorporated into 30μM modified crosstide peptide (GRPRTSSFAEGKK) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 43 kDa
• AA Sequence: 1 MHHHHHHMNC SPTSQIDNIG EEEMDASTTH HKRKTMNDFD YLKLLGKGTF GKVILVREKA 61 SGKYYAMKIL KKEVIIAKDE VAHTLTESRV LKNTRHPFLT SLKYSFQTKD RLCFVMEYVN 121 GGELFFHLSR ERVFSEDRTR FYGAEIVSAL DYLHSGKIVY RDLKLENLML DKDGHIKITD 181 FGLCKEGITD AATMKTFCGT PEYLAPEVLE DNDYGRAVDW WGLGVVMYEM MCGRLPFYNQ 241 DHEKLFELIL MEDIKFPRTL SSDAKSLLSG LLIKDPNKRL GGGPDDAKEI MRHSFFSGVN 301 WQDVYDKKLV PPFKPQVTSE TDTRYFDEEF TAQTITITPP EKYDEDGMDC MDNERRPHFP 361 QFDYSASGRE
• Purity: Purity 92% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: AKT3 AKT serine/threonine kinase 3 [ Homo sapiens (human) ]
• Official Symbol: AKT3
• Synonyms: AKT3; AKT serine/threonine kinase 3; MPPH; PKBG; MPPH2; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma
• Gene ID: 10000
• mRNA Refseq: NM_001206729.2
• Protein Refseq: NP_001193658.1
• MIM: 611223
• UniProt ID: Q9Y243