Active Recombinant Human TYK2(875-end) Protein, N-His-tagged

• Cat.No.: TYK2-253H
• Product Overview: N-terminal 6His-tagged, recombinant, human TYK2 amino acids 875- end, expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography followed by gel filtration.

Specification

• Description: TYK2 is member of the tyrosine kinase and, more specifically, the Janus kinases (JAKs) protein families. This protein associates with the cytoplasmic domain of type I and type II cytokine receptors and promulgate cytokine signals by phosphorylating receptor subunits. It is also component of both the type I and type III interferon signaling pathways. As such, it may play a role in anti-viral immunity. A mutation in this gene has been associated with hyperimmunoglobulin E syndrome (HIES) - a primary immunodeficiency characterized by elevated serum immunoglobulin E.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 875-end
• Form: 0.175mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 65U/mg, where one unit of TYK2, active activity is defined as 1nmol phosphate incorporated into 250μM (GGMEDIYFEFMGGKKK) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 40 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF ADVLTVNPDS PASDPTVFHK RYLKKIRDLG 61 EGHFGKVSLY CYDPTNDGTG EMVAVKALKA DCGPQHRSGW KQEIDILRTL YHEHIIKYKG 121 CCEDQGEKSL QLVMEYVPLG SLRDYLPRHS IGLAQLLLFA QQICEGMAYL HSQHYIHRDL 181 AARNVLLDND RLVKIGDFGL AKAVPEGHEY YRVREDGDSP VFWYAPECLK EYKFYYASDV 241 WSFGVTLYEL LTHCDSSQSP PTKFLELIGI AQGQMTVLRL TELLERGERL PRPDKCPCEV 301 YHLMKNCWET EASFRPTFEN LIPILKTVHE KYQGQAPSVF SVC
• Purity: Purity 78.5% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: TYK2 tyrosine kinase 2 [ Homo sapiens (human) ]
• Official Symbol: TYK2
• Synonyms: TYK2; tyrosine kinase 2; JTK1; IMD35
• Gene ID: 7297
• mRNA Refseq: NM_001385197.1
• Protein Refseq: NP_001372126.1
• MIM: 176941
• UniProt ID: P29597

Reviews

10/14/2022

Their expertise in protein production and purification ensures the delivery of a high-quality product that consistently performs well in my experiments.

06/15/2022

They are readily available to address any concerns or questions and supply valuable resources to assist in experimental design and optimization.

01/24/2018

Should I encounter any challenges or require technical assistance, the manufacturer provides prompt and knowledgeable support.

Q&As

Could TYK2 inhibitors have applications beyond autoimmune diseases?

Yes, TYK2 inhibitors might have potential in treating certain cancers and possibly other inflammatory conditions beyond autoimmune diseases.

What are the potential benefits of targeting TYK2 in treating autoimmune diseases?

TYK2 inhibition can potentially reduce inflammation and modulate immune responses without suppressing the entire immune system, unlike some other treatments.

Are there any clinical trials ongoing or completed regarding TYK2 inhibitors?

Yes, there are several clinical trials evaluating the efficacy and safety of TYK2 inhibitors in various autoimmune diseases.

How do TYK2 inhibitors differ from other treatments for autoimmune diseases?

TYK2 inhibitors are more targeted, aiming to interfere with specific signaling pathways involved in the immune response compared to broad immunosuppressants.

How are TYK2 inhibitors administered to patients?

Depending on the formulation, TYK2 inhibitors can be administered orally or through injections.