"UBE2F" Related Products

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Recombinant Human Ubiquitin-Conjugating Enzyme E2F (putative), His-tagged

Cat.No.: UBE2F-2484H
Product Overview: Recombinant human UBE2F protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Description: UBE2F belongs to the ubiquitin-conjugating enzyme family and UBE2F subfamily. Ubiquitin-conjugating enzymes more rarely as ubiquitin-carrier enzymes, perform the second step in the ubiquitination reaction that targets a protein for degradation via the proteasome. UBE2F accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins.
Source: E. coli
Species: Human
Tag: His
Form: Liquid. In 20 mM Tris-HCl buffer, pH8.0, 30% glycerol, 1mM DTT, 200mM NaCl.
Molecular Mass: 23.7kDa (209aa) confirmed by MALDI-TOF
Protein length: 1-185aa
AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMLTLAS KLKRDDGLKG SRTAATASDS TRRVSVRDKL LVKEVAELEA NLPCTCKVHF PDPNKLHCFQ LTVTPDEGYY QGGKFQFETE VPDAYNMVPP KVKCLTKIWH PNITETGEIC LSLLREHSID GTGWAPTRTL KDVVWGLNSL FTDLLNFDDP LNIEAAEHHL RDKEDFRNKV DDYIKRYAR
Purity: >95% by SDS - PAGE
Applications: SDS-PAGE
Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -78°C. Avoid repeated freezing and thawing cycles.
Concentration: 1mg/ml (determined by Bradford assay)
Gene Name: UBE2F ubiquitin-conjugating enzyme E2F (UBC6 homolog, C. elegans) [ Homo sapiens ]
Official Symbol: UBE2F
Synonyms: UBE2F; ubiquitin-conjugating enzyme E2F (UBC6 homolog, C. elegans); NCE2; NEDD8 conjugating enzyme;
Gene ID: 23665
UniProt ID: Q969M7
Chromosome Location: 2q37.3
Pathway: Cell cycle, organism-specific biosystem;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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