"UBE2V2" Related Products

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Recombinant Human UBE2V2 293 Cell Lysate

Cat.No.: UBE2V2-559HCL
Description: Antigen standard for ubiquitin-conjugating enzyme E2 variant 2 (UBE2V2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: UBE2V2 ubiquitin-conjugating enzyme E2 variant 2 [ Homo sapiens ]
Official Symbol: UBE2V2
Synonyms: UBE2V2; ubiquitin-conjugating enzyme E2 variant 2; DDVit 1; EDPF 1; MMS2; UEV 2; MMS2 homolog; vitamin D3-inducible protein; 1 alpha,25-dihydroxyvitamin D3-inducible; enterocyte differentiation promoting factor; enterocyte differentiation-promoting factor 1; enterocyte differentiation-associated factor 1; enterocyte differentiation-associated factor EDAF-1; methyl methanesulfonate sensitive 2, S. cerevisiae, homolog of; UEV2; EDPF1; UEV-2; DDVIT1; EDAF-1; EDPF-1; DDVit-1;
Gene ID: 7336
mRNA Refseq: NM_003350
Protein Refseq: NP_003341
MIM: 603001
UniProt ID: Q15819
Chromosome Location: 8q11.21
Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Immune System, organism-specific biosystem;

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