Active Recombinant Human RAD51

• Cat.No.: RAD51-1121H

Specification

• Cat. No.: RAD51-1121H
• Description: Properly controlled recombination between homologous DNA molecules (Homologous Recombination -HR) is essential for the maintenance of genome stability and for the prevention of tumorigenesis. Rad51 is a mammalian homologue of yeast RAD51 and bacterial RecA and, like its counterparts, plays a central role in HR. Rad51 coats ssDNA to form a nucleoprotein filament that invades and pairs with a homologous region in duplex DNA. It can then activate strand exchange to generate a crossover between the juxtaposed DNA molecules. In addition to Rad51, these steps require the coordinated action of a number of other homologous-recombination proteins, including the RP-A protein, which binds single-stranded DNA, Rad52, which can bind DNA ends, anneal complementary single-stranded DNA molecules and enhance the specificity of RAD51, and a number of Rad51 paralogs. The tumour-suppressor proteins BRCA1 and BRCA2 colocalize with RAD51 in DNA-damage-induced nulear foci. BRCA2 has been shown to interact directly with RAD51 and thus plays a direct role in repair by HR, through control of the availability and function of the central mediator, Rad51. Recombinant RAD51 was expressed in a strain of E.coli and purified by an affinity column in combination with FPLC chromatography.
• Source: E.coli.
• Form: Liquid. Supplied in 20 mM Tris-HCl pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT and 20% glycerol.
• Purity: ≥95% by SDS-PAGE.
• Application: RAD51 has been applied in DNA and protein-protein interaction assays.
• Activity: 20 ng are sufficient for a DNA-protein assay and 100 ng are sufficient for a protein-protein interaction assay.
• Usage: For in vitro use only.
• Storage: Quality guaranteed for 12 months store at -80°C. Avoid freeze / thaw cycles.

Gene Information

• Gene Name: RAD51 RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) [ Homo sapiens ]
• Synonyms: RAD51; RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae); RECA; BRCC5; HRAD51; RAD51A; HsRad51; HsT16930; RAD51 homolog protein; RecA-like protein; RAD51D; recombination protein A; RecA, E. coli, homolog of DNA repair protein RAD51 homolog 1; BRCA1/BRCA2-containing complex, subunit 5; RAD51L3; hRAD51; RAD51 (S. cerevisiae) homolog (E coli RecA homolog)
• Gene ID: 5888
• mRNA Refseq: NM_002875
• Protein Refseq: NP_002866
• MIM: 179617
• UniProt ID: Q06609
• Chromosome Location: 15q15
• Pathway: Homologous recombination; Pancreatic cancer; Pathways in cancer
• Function: ATP binding; damaged DNA binding; double-stranded DNA binding; identical protein binding; nucleoside-triphosphatase activity; nucleotide binding; protein C-terminus binding; single-stranded DNA binding; single-stranded DNA-dependent ATPase activity

Reviews

04/02/2024

RAD51-134H, Rad51 works well.

09/05/2022

User-friendly protocol included.

03/14/2022

Affordable price, great value.

01/03/2022

Product met all specifications.

Q&As

How does RAD51 contribute to the homologous recombination (HR) repair pathway?

In the HR pathway, RAD51 forms nucleoprotein filaments on single-stranded DNA, allowing it to search for and invade a homologous DNA sequence, facilitating accurate DNA repair.

Are there any known interactions between RAD51 and BRCA proteins?

Yes, RAD51 interacts with BRCA proteins, especially BRCA2. This interaction is crucial for the proper function of RAD51 in the HR pathway.

Are there any diseases or conditions associated with RAD51 dysregulation or mutations?

While mutations in RAD51 are rare, dysregulation of its function can contribute to genomic instability. Some mutations in RAD51 have been linked to increased cancer susceptibility.

What is the primary function of RAD51 in DNA repair mechanisms?

RAD51 plays a central role in the DNA repair mechanism, specifically in homologous recombination (HR), where it facilitates the search for homology and strand pairing.

How is RAD51 recruited to sites of DNA damage?

RAD51 is recruited to sites of DNA damage through interactions with various mediator proteins, including BRCA2, which helps load RAD51 onto single-stranded DNA regions.

Can RAD51 activity be targeted therapeutically in cancer treatments?

Yes, RAD51's activity can be targeted therapeutically. Inhibiting RAD51 can sensitize cancer cells to DNA-damaging agents, making it a potential target in combination cancer therapies, especially for tumors with defective DNA repair pathways.

How does RAD51's function compare to that of its paralogs, like RAD51B, RAD51C, etc.?

RAD51 has several paralogs, including RAD51B, RAD51C, RAD51D, XRCC2, and XRCC3. While they all play roles in HR, each has distinct functions and interactions, with some acting as mediators or stabilizers of RAD51's nucleoprotein filament.