Active Recombinant Full Length Human DUSP22 Protein, N-GST-tagged

• Cat.No.: DUSP22-67HFL
• Product Overview: N-terminal GSTtagged, recombinant, human DUSP22, full length, expressed in E. coli cells. Purified using glutathione agarose.

Specification

• Description: Enables non-membrane spanning protein tyrosine phosphatase activity and protein tyrosine kinase binding activity. Involved in several processes, including cellular response to epidermal growth factor stimulus; negative regulation of focal adhesion assembly; and negative regulation of non-membrane spanning protein tyrosine kinase activity. Acts upstream of or within negative regulation of transcription by RNA polymerase II. Located in plasma membrane. Part of cytoplasm; filamentous actin; and leading edge of lamellipodium.
• Source: E. coli
• Species: Human
• Tag: GST
• Protein length: Full Length
• Form: 1.629mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 360U/mg, where one unit of DUSP22, active activity is defined as the release of 1nmol of phosphate per minute from the phosphorylated substrate 6,8-difluoro-4- methylumbelliferyl phosphate (DiFMUP) at room temperature.
• Molecular Mass: 47.3 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSMGNG MNKILPGLYI 241 GNFKDARDAE QLSKNKVTHI LSVHDSARPM LEGVKYLCIP AADSPSQNLT RHFKESIKFI 301 HECRLRGESC LVHCLAGVSR SVTLVIAYIM TVTDFGWEDA LHTVRAGRSC ANPNVGFQRQ 361 LQEFEKHEVH QYRQWLKEEY GESPLQDAEE AKNILAAPGI LKFWAFLRRL
• Purity: Purity 91.9% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: DUSP22 dual specificity phosphatase 22 [ Homo sapiens (human) ]
• Official Symbol: DUSP22
• Synonyms: DUSP22; dual specificity phosphatase 22; VHX; JKAP; JSP1; MKPX; JSP-1; MKP-x; LMWDSP2; LMW-DSP2
• Gene ID: 56940
• mRNA Refseq: NM_020185.6
• Protein Refseq: NP_064570.1
• MIM: 616778
• UniProt ID: Q9NRW4