Active Recombinant Full Length Human PIK3C3 Protein, N-His-tagged

• Cat.No.: PIK3C3-144HFL
• Product Overview: N-terminal 6Histagged, recombinant, human PIK3C3 full length, expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography

Specification

• Description: Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways. PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. As part of PI3KC3-C1, promotes endoplasmic reticulum membrane curvature formation prior to vesicle budding. Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2. Involved in the transport of lysosomal enzyme precursors to lysosomes (By similarity). Required for transport from early to late endosomes (By similarity). (Microbial infection) Kinase activity is required for SARS coronavirus-2/SARS-CoV-2 replication
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 2.110mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol, 20% glycerol. Frozen solution.
• Bio-activity: 242U/mg, where one unit of PIK3C3, active activity is defined as 1nmol phosphatidylinositol 3-phosphate (PI(3)P) formed per minute at room temperature with a final ATP concentration of 200μM.
• Molecular Mass: 105 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MGEAEKFHYI YSCDLDINVQ LKIGSLEGKR 61 EQKSYKAVLE DPMLKFSGLY QETCSDLYVT CQVFAEGKPL ALPVRTSYKA FSTRWNWNEW 121 LKLPVKYPDL PRNAQVALTI WDVYGPGKAV PVGGTTVSLF GKYGMFRQGM HDLKVWPNVE 181 ADGSEPTKTP GRTSSTLSED QMSRLAKLTK AHRQGHMVKV DWLDRLTFRE IEMINESEKR 241 SSNFMYLMVE FRCVKCDDKE YGIVYYEKDG DESSPILTSF ELVKVPDPQM SMENLVESKH 301 HKLARSLRSG PSDHDLKPNA ATRDQLNIIV SYPPTKQLTY EEQDLVWKFR YYLTNQEKAL 361 TKFLKCVNWD LPQEAKQALE LLGKWKPMDV EDSLELLSSH YTNPTVRRYA VARLRQADDE 421 DLLMYLLQLV QALKYENFDD IKNGLEPTKK DSQSSVSENV SNSGINSAEI DSSQIITSPL 481 PSVSSPPPAS KTKEVPDGEN LEQDLCTFLI SRACKNSTLA NYLYWYVIVE CEDQDTQQRD 541 PKTHEMYLNV MRRFSQALLK GDKSVRVMRS LLAAQQTFVD RLVHLMKAVQ RESGNRKKKN 601 ERLQALLGDN EKMNLSDVEL IPLPLEPQVK IRGIIPETAT LFKSALMPAQ LFFKTEDGGK 661 YPVIFKHGDD LRQDQLILQI ISLMDKLLRK ENLDLKLTPY KVLATSTKHG FMQFIQSVPV 721 AEVLDTEGSI QNFFRKYAPS ENGPNGISAE VMDTYVKSCA GYCVITYILG VGDRHLDNLL 781 LTKTGKLFHI DFGYILGRDP KPLPPPMKLN KEMVEGMGGT QSEQYQEFRK QCYTAFLHLR 841 RYSNLILNLF SLMVDANIPD IALEPDKTVK KVQDKFRLDL SDEEAVHYMQ SLIDESVHAL 901 FAAVVEQIHK FAQYWRK
• Purity: Purity 90% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PIK3C3 phosphatidylinositol 3-kinase catalytic subunit type 3 [ Homo sapiens (human) ]
• Official Symbol: PIK3C3
• Synonyms: PIK3C3; phosphatidylinositol 3-kinase catalytic subunit type 3; VPS34; Vps34; hVps34
• Gene ID: 5289
• mRNA Refseq: NM_002647.4
• Protein Refseq: NP_002638.2
• MIM: 602609
• UniProt ID: Q8NEB9