Recombinant Mouse Pik3c3 Protein, Myc/DDK-tagged

• Cat.No.: Pik3c3-4863M
• Product Overview: Purified recombinant protein of mouse full-length phosphoinositide-3-kinase, class 3 (cDNA clone MGC:30377 IMAGE:3661094), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes.
• Molecular Mass: 96.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Pik3c3 phosphatidylinositol 3-kinase catalytic subunit type 3 [ Mus musculus (house mouse) ]
• Official Symbol: Pik3c3
• Synonyms: Pik3c3; phosphatidylinositol 3-kinase catalytic subunit type 3; Vp; Vps34; 5330434F23Rik; phosphatidylinositol 3-kinase catalytic subunit type 3; PI3-kinase type 3; PI3K type 3; phosphoinositide-3-kinase, class 3; ptdIns-3-kinase type 3; EC 2.7.1.137
• Gene ID: 225326
• mRNA Refseq: NM_181414
• Protein Refseq: NP_852079
• UniProt ID: Q6PF93