Active Recombinant Full Length Human PPP5C Protein, N-GST-tagged

• Cat.No.: PPP5C-160HFL
• Product Overview: N-terminal GSTtagged, recombinant, full length, human PP5, expressed in E. coli cells. Purified using glutathione agarose.

Specification

• Description: PP5 is a serine/threonine phosphatase which is a member of the protein phosphatase catalytic subunit family. Proteins in this family participate in pathways regulated by reversible phosphorylation at serine and threonine residues; many of these pathways are involved in the regulation of cell growth and differentiation. The product of this gene has been shown to participate in signaling pathways in response to hormones or cellular stress, and elevated levels of this protein may be associated with breast cancer development. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: GST
• Protein length: Full Length
• Form: 1.453mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 23U/mg, where one unit of PP5, active activity is defined as the release of 1nmol of phosphate per minute from the phosphorylated substrate 6,8-difluoro-4- methylumbelliferyl phosphate (DiFMUP) at room temperature.
• Molecular Mass: 83.8 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPLG SMAMAEGERT 241 ECAEPPRDEP PADGALKRAE ELKTQANDYF KAKDYENAIK FYSQAIELNP SNAIYYGNRS 301 LAYLRTECYG YALGDATRAI ELDKKYIKGY YRRAASNMAL GKFRAALRDY ETVVKVKPHD 361 KDAKMKYQEC NKIVKQKAFE RAIAGDEHKR SVVDSLDIES MTIEDEYSGP KLEDGKVTIS 421 FMKELMQWYK DQKKLHRKCA YQILVQVKEV LSKLSTLVET TLKETEKITV CGDTHGQFYD 481 LLNIFELNGL PSETNPYIFN GDFVDRGSFS VEVILTLFGF KLLYPDHFHL LRGNHETDNM 541 NQIYGFEGEV KAKYTAQMYE LFSEVFEWLP LAQCINGKVL IMHGGLFSED GVTLDDIRKI 601 ERNRQPPDSG PMCDLLWSDP QPQNGRSISK RGVSCQFGPD VTKAFLEENN LDYIIRSHEV 661 KAEGYEVAHG GRCVTVFSAP NYCDQMGNKA SYIHLQGSDL RPQFHQFTAV PHPNVKPMAY 721 ANTLLQLGMM
• Purity: Purity 78.6% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PPP5C protein phosphatase 5 catalytic subunit [ Homo sapiens (human) ]
• Official Symbol: PPP5C
• Synonyms: PPP5C; protein phosphatase 5 catalytic subunit; PP5; PPT; PPP5
• Gene ID: 5536
• mRNA Refseq: NM_006247.4
• Protein Refseq: NP_006238.1
• MIM: 600658
• UniProt ID: P53041