| Description : |
RSK4 (Ribosomal S6 kinase 4) is a serine/threonine protein kinase that belongs to the RSK (Ribosomal S6 kinase) family of kinases. It is involved in a variety of cellular processes, including cell growth and differentiation, transcriptional regulation, and cytoskeletal dynamics. Research has suggested that RSK4 plays a role in several diseases, including cancer and X-linked mental retardation. Mutations in the RSK4 gene have been linked to Coffin-Lowry syndrome, a rare genetic disorder characterized by intellectual disability, facial abnormalities, and other physical features. Additionally, RSK4 has been shown to be involved in the regulation of insulin signaling and glucose metabolism, making it a potential target for the treatment of type 2 diabetes. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
Full Length |
| Form : |
0.206mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
255U/mg, where one unit of Rsk4, active activity is defined as 1nmol phosphate incorporated into PAKtide per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
85.2 kDa |
| AA Sequence : |
1 MHHHHHHEFM LPFAPQDEPW DREMEVFSGG GASSGEVNGL KMVDEPMEEG EADSCHDEGV 61 VKEIPITHHV KEGYEKADPA QFELLKVLGQ GSFGKVFLVR KKTGPDAGQL YAMKVLKKAS 121 LKVRDRVRTK MERDILVEVN HPFIVKLHYA FQTEGKLYLI LDFLRGGDVF TRLSKEVLFT 181 EEDVKFYLAE LALALDHLHQ LGIVYRDLKP ENILLDEIGH IKLTDFGLSK ESVDQEKKAY 241 SFCGTVEYMA PEVVNRRGHS QSADWWSYGV LMFEMLTGTL PFQGKDRNET MNMILKAKLG 301 MPQFLSAEAQ SLLRMLFKRN PANRLGSEGV EEIKRHLFFA NIDWDKLYKR EVQPPFKPAS 361 GKPDDTFCFD PEFTAKTPKD SPGLPASANA HQLFKGFSFV ATSIAEEYKI TPITSANVLP 421 IVQINGNAAQ FGEVYELKED IGVGSYSVCK RCIHATTNME FAVKIIDKSK RDPSEEIEIL 481 MRYGQHPNII TLKDVFDDGR YVYLVTDLMK GGELLDRILK QKCFSEREAS DILYVISKTV 541 DYLHCQGVVH RDLKPSNILY MDESASADSI RICDFGFAKQ LRGENGLLLT PCYTANFVAP 601 EVLMQQGYDA ACDIWSLGVL FYTMLAGYTP FANGPNDTPE EILLRIGNGK FSLSGGNWDN 661 ISDGAKDLLS HMLHMDPHQR YTAEQILKHS WITHRDQLPN DQPKRNDVSH VVKGAMVATY 721 SALTHKTFQP VLEPVAASSL AQRRSMKKRT STGL |
| Purity : |
Purity 80.9% by SDSPAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
