RPS6KA1

  • Official Full Name

    ribosomal protein S6 kinase, 90kDa, polypeptide 1

  • Overview

    RSK 1 (p90-RSK 1) is a member of a family of 90 kDa ribosomal protein S6 kinases, which includes RSK 1, RSK 2 and RSK 3. These are broadly expressed serine/threonine protein kinases (1) activated in response to mitogenic stimuli, including extracellular signal-regulated protein kinases Erk1 and Erk2 (2). p90-RSK 1 is activated by MAPK in vitro and in vivo via phosphorylation (3). Active RSKs appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB (2,4).
  • Synonyms

    RPS6KA1; ribosomal protein S6 kinase, 90kDa, polypeptide 1; ribosomal protein S6 kinase, 90kD, polypeptide 1; ribosomal protein S6 kinase alpha-1; HU 1; RSK; RSK1; RSK-1; p90S6K; p90RSK1; p90-RSK 1; MAPKAPK-1a; S6K-alpha 1; S6K-alpha-1; MAPKAP kinase 1a; ribosomal S6 kinase 1; MAPK-activated protein kinase 1a; ribosomal protein S6 kinase alpha 1; 90 kDa ribosomal protein S6 kinase 1; MAP kinase-activated protein kinase 1a; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1); HU-1; MAPKAPK1A;

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Protein Pre-coupled Magnetic Beads
  • Chicken
  • Human
  • Human
  • Mouse
  • Rat
  • Zebrafish
  • E.coli
  • HEK293
  • HEK293T
  • Insect (sf21)
  • Insect Cell
  • Mammalian Cell
  • Mammalian cells
  • Sf21
  • Sf9 Insect Cell
  • C
  • His
  • GST
  • His (Fc)
  • Avi
  • His,HA
  • His|T7
  • Myc
  • DDK
  • N/A
  • N
  • No tag
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human RPS6KA1-555H Active Recombinant Human RPS6KA1, GST-tagged Sf9 Insect Cell GST
Human RPS6KA1-167HFL Active Recombinant Full Length Human RPS6KA1 Protein, N-His-tagged Sf21 His Full Length
Human RPS6KA1-30110TH Recombinant Human RPS6KA1 N/A
Human RPS6KA1-550H Recombinant Human RPS6KA1 Mammalian Cell His
Human RPS6KA1-424H Recombinant Human RPS6KA1, GST-tagged, Active Sf9 Insect Cell GST
Human RPS6KA1-2432H Recombinant Human RPS6KA1, GST-tagged E.coli GST 432-735aa
Human RPS6KA1-2162HCL Recombinant Human RPS6KA1 293 Cell Lysate HEK293 N/A
Human RPS6KA1-3984H Recombinant Human RPS6KA1 protein, His-tagged E.coli His 432-735 aa
Human RPS6KA1-1934H Recombinant Human RPS6KA1 protein, His & T7-tagged E.coli His/T7 Phe62~Tyr321 (Accession # Q15418)
Human RPS6KA1-4414H Recombinant Human RPS6KA1 Protein, Myc/DDK-tagged, C13 and N15-labeled HEK293T Myc/DDK
Human RPS6KA1-1148H Recombinant Human RPS6KA1 Protein (Q33-T353), Tag Free Insect Cell No tag Q33-T353
Human RPS6KA1-6201H Recombinant Human RPS6KA1 Protein (Phe62-Tyr321), N-His tagged E.coli N-His Phe62-Tyr321
Human RPS6KA1-4010H Recombinant Human RPS6KA1 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Human RPS6KA1-8403HF Active Recombinant Full Length Human RPS6KA1 Protein, GST-tagged Insect (sf21) GST 1-735
Human RPS6KA1-4010H-B Recombinant Human RPS6KA1 Protein Pre-coupled Magnetic Beads HEK293
Human RPS6KA1-6200H Recombinant Human RPS6KA1 Protein (Met1-Leu735), C-His tagged Mammalian cells C-His Met1-Leu735
Human RPS6KA1-1149H Recombinant Human RPS6KA1 Protein (Q33-T353), His tagged Insect Cell His Q33-T353
Mouse Rps6ka1-5609M Recombinant Mouse Rps6ka1 Protein, Myc/DDK-tagged HEK293T Myc/DDK
Rat RPS6KA1-166RFL Active Recombinant Full Length Rat RPS6KA1 Protein, N-His,HA-tagged Sf21 His,HA Full Length
Rat RPS6KA1-5172R Recombinant Rat RPS6KA1 Protein Mammalian Cell His
Rat RPS6KA1-4831R-B Recombinant Rat RPS6KA1 Protein Pre-coupled Magnetic Beads HEK293
Rat RPS6KA1-4831R Recombinant Rat RPS6KA1 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Zebrafish RPS6KA1-4852Z Recombinant Zebrafish RPS6KA1 Mammalian Cell His
Chicken RPS6KA1-3760C Recombinant Chicken RPS6KA1 Mammalian Cell His
  • Background
  • Quality Guarantee
  • Case Study
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • Other Resource
  • RPS6KA1 Related Signal Pathway

What is RPS6KA1 protein?

RPS6KA1 (ribosomal protein S6 kinase A1) gene is a protein coding gene which situated on the short arm of chromosome 1 at locus 1p36. This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. The RPS6KA1 protein is consisted of 735 amino acids and its molecular mass is approximately 82.7 kDa.

What is the function of RPS6KA1 protein?

RPS6KA1 promotes cell growth, proliferation and survival by activating the mTORC1 signaling pathway. RPS6KA1 can influence the process of ribosomal protein synthesis and translation, thus affecting the synthesis of proteins within the cell. RPS6KA1 plays a role in the G1 and S phases of the cell cycle and regulates cell cycle progression. RPS6KA1 maintains cell survival by inhibiting cell stress response and apoptosis pathway.

RPS6-9.jpg

Fig1. Regulation of the cell-cycle by RSK. (Rana Anjum, 2008)

RPS6KA1 Related Signaling Pathway

RPS6KA1 is one of the key downstream effectors of the MAPK/ERK (mitogen-activated protein kinase/Extracellular signal-regulated kinase) pathway, through which it is involved in signal transduction induced by various growth factors and hormones. The PI3K/AKT signaling pathway plays a key role in cell survival, metabolism, and migration, and RPS6KA1, as a component of this pathway, responds to PI3K (phosphoinositol-3-kinase) /AKT activation, which in turn influences downstream events. RPS6KA1 is involved in regulating the G1 to S phase transition, ensuring that cells are ready before DNA replication.

RPS6-10.jpg

Fig2. A schematic model for RSK activation. (Rana Anjum, 2008)

RPS6KA1 Related Diseases

Overactivation of RPS6KA1 plays a role in the development of many cancers, including breast cancer, pancreatic cancer, liver cancer, and neuroblastoma. The expression and activation of RPS6KA1 in cardiomyocytes is associated with cardioprotective mechanisms, but its dysregulation may also be associated with the development of cardiovascular diseases. RPS6KA1 plays a role in signaling in neurons, and abnormalities in it may be associated with neurodegenerative diseases such as Alzheimer's disease. It's also associated with diabetes.

Bioapplications of RPS6KA1

RPS6KA1 is abnormally expressed in a variety of cancers, including prostate, breast and pancreatic cancers. Therefore, it is considered a potential cancer therapeutic target, and researchers are exploring small molecule drugs that inhibit RPS6KA1 activity or its signaling pathway for tumor therapy.

Case Study 1: Kazuhiro Katayama, 2016

P-glycoprotein (P-gp) is a critical determinant of multidrug resistance in cancer. This team previously reported that MAPK inhibition downregulates P-gp expression and that P-gp undergoes ubiquitin-proteasomal degradation regulated by UBE2R1 and SCFFbx15. Here, the researchers investigated the crosstalk between MAPK inhibition and the ubiquitin-proteasomal degradation of P-gp. Proteasome inhibitors or knockdown of FBXO15 and/or UBE2R1 cancelled MEK inhibitor-induced P-gp downregulation. RSK1 phosphorylated Thr162 on UBE2R1 but did not phosphorylate FBXO15. MEK and RSK inhibitors increased UBE2R1-WT but not UBE2R1-T162D and -T162A expression. UBE2R1-T162D showed higher self-ubiquitination and destabilisation than UBE2R1-WT and -T162A. Unlike UBE2R1-WT and -T162A, UBE2R1-T162D did not induce P-gp ubiquitination. UBE2R1-WT or -T162A downregulated P-gp expression and upregulated rhodamine 123 level and sensitivity to vincristine and doxorubicin. However, UBE2R1-T162D did not confer any change in P-gp expression, rhodamine 123 accumulation and sensitivity to the drugs.

RPS6-3.jpg

Fig1. Binding of ERK1 and RSK1 with FBXO15.

RPS6-4.jpg
Fig2. RSK1-mediated phosphorylation of UBE2R1 in transfectants.

Case Study 2: Amel Salhi, 2015

The two major melanoma histologic subtypes, superficial spreading and nodular melanomas, differ in their speed of dermal invasion but converge biologically once they invade and metastasize. Herein, the researchers tested the hypothesis that distinct molecular alterations arising in primary melanoma cells might persist as these tumors progress to invasion and metastasis. Ribosomal protein S6 kinase, 90 kDa, polypeptide 1 (RSK1; official name RPS6KA1) was significantly hyperactivated in human melanoma lines and metastatic tissues derived from nodular compared with superficial spreading melanoma. RSK1 was constitutively phosphorylated at Ser-380 in nodular but not superficial spreading melanoma and did not directly correlate with BRAF or MEK activation. Nodular melanoma cells were more sensitive to RSK1 inhibition using siRNA and the pharmacological inhibitor BI-D1870 compared with superficial spreading cells. Gene expression microarray analyses revealed that RSK1 orchestrated a program of gene expression that promoted cell motility and invasion. Differential overexpression of the prometastatic matrix metalloproteinase 8 and tissue inhibitor of metalloproteinases 1 in metastatic nodular compared with metastatic superficial spreading melanoma was observed. Finally, using an in vivo zebrafish model, constitutive RSK1 activation increased melanoma invasion.

RPS6-5.jpg

Fig3. p-RSK1 protein expression in human metastatic melanoma tissues by Western blot analysis.

RPS6-6.jpg
Fig4. Western blot analysis showing RSK1 levels after transfection with nontargeting.
RPS6-7.jpg

Fig1. Harmonization of Ca2+, EGF, and oxidative signals. (Tsuyoshi Takata, 2020)

RPS6-8.jpg

Fig2. Mitogen- and stress-activated kinases (MSKs). (Rana Anjum, 2008)

RPS6KA1 involved in several pathways and played different roles in them. We selected most pathways RPS6KA1 participated on our site, such as MAPK signaling pathway, Oocyte meiosis, mTOR signaling pathway, which may be useful for your reference. Also, other proteins which involved in the same pathway with RPS6KA1 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
MAPK signaling pathwayTP53;FGF12A;FGFR1A;PRKACB;FGF20B;PTPRR;MAP2K4B;NTF3;MAPK11
Oocyte meiosisFBXW11;SMC1AL;RPS6KA3A;PTTG2;ESPL1;INS1;ADCY1B;CCNB2;CAMK2B
mTOR signaling pathwayPRKCB;DDIT4;HIF1A;PIK3R3B;ULK3;EIF4BA;RPS6;TSC1B;RICTOR
Long-term potentiationGRIN1;RPS6KA2;RPS6KA1;GNAQ;PRKACG;PRKACB;PRKACA;CALML3;NRAS
Neurotrophin signaling pathwayMAPK13;GRB2;CAMK4;SH2B2;NTRK1;NGFRAP1;PRKCD;SHC2;MAP3K3
Progesterone-mediated oocyte maturationPRKACG;MAPK10;PIK3CD;CDC25C;PPP1R2P9;CDC25A;CDC25B;CCNA2;PRKACBA
Insulin resistanceSLC27A2;PDPK1B;SOCS3B;PTPRF;PTPRFB;PPARGC1A;RPS6KA3A;PRKAB2;IKBKB

RPS6KA1 has several biochemical functions, for example, ATP binding, cysteine-type endopeptidase inhibitor activity involved in apoptotic process, magnesium ion binding. Some of the functions are cooperated with other proteins, some of the functions could acted by RPS6KA1 itself. We selected most functions RPS6KA1 had, and list some proteins which have the same functions with RPS6KA1. You can find most of the proteins on our site.

Function Related Protein
ATP bindingXRCC2;DTYMK;PDGFRA;PRKAA1;NLRP4C;MYH10;EPHB1;ACSBG1;AACS
cysteine-type endopeptidase inhibitor activity involved in apoptotic processIGF1RA;BIRC5;LEF1;VIL1;SNCA;RPS6KA1;BIRC2;PRDX3;ARRB1
magnesium ion bindingCDC42BPA;LATS1;TDP2B;WEE2;TOP2A;PRPS1L1;ENO2;MAST4;PAPOLA
protein bindingEPS15L1;CD276;EID3;CCDC88A;Trl;KCNRG;PTRH2;PTGIS;PCDHA4
protein serine/threonine kinase activitySRPK2;AURKA;CDK15;SBK3;ULK4;RPS6KA5;PIM3;MAP4K4;GRK7B
protein serine/threonine/tyrosine kinase activityDYRK4;RPS6KB1;TESK2;TTK;AKT1;PRKAA2;AURKB;DYRK1B;PRKCG
ribosomal protein S6 kinase activityRPS6KA1;RPS6KA3A;ALPK2;RPS6KB1B;RPS6KB2;RPS6KA3;RPS6KA6;RPS6KA2;RPS6KB1

RPS6KA1 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with RPS6KA1 here. Most of them are supplied by our site. Hope this information will be useful for your research of RPS6KA1.

HSP90AB1; VASP; MAPT; CREB1; EIF4B; RPS6; xmd8-92; biotin_acyl-p_probe; YWHAB; ENAH; PPM1G; DDAH2; CFL1; GBP2; TTK; CCT4

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Customer Reviews (3)

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Reviews
07/10/2022

    Exceptional protein purification, highly recommended for research.

    06/02/2022

      Dependable mass spectrometry analysis; enhances our proteomics research.

      05/26/2020

        Quick and precise protein expression, a valuable service.

        Q&As (7)

        Ask a question
        What is the impact of RPS6KA1 dysregulation on metabolic disorders, such as diabetes and obesity? 10/29/2020

        Dysregulation of RPS6KA1 impacts metabolic processes, influencing conditions like diabetes and obesity.

        What are the mechanisms by which RPS6KA1 modulates the cell cycle and apoptosis? 01/27/2020

        RPS6KA1 modulates the cell cycle and apoptosis, playing a role in cell proliferation and programmed cell death.

        How does RPS6KA1 influence neuronal development and function, especially in learning and memory processes? 07/20/2019

        RPS6KA1 is involved in neuronal development and function, affecting processes like learning and memory.

        How do genetic mutations in RPS6KA1 contribute to the development of certain cancers and other diseases? 06/24/2019

        Genetic mutations in RPS6KA1 are linked to certain cancers and diseases, affecting its kinase activity and signaling functions.

        How does RPS6KA1 interact with other kinases and signaling molecules in cellular signaling networks? 03/25/2019

        RPS6KA1 interacts with various kinases and signaling molecules, integrating multiple cellular signaling pathways.

        What role does RPS6KA1 play in cellular responses to stress, particularly in cancer cells? 09/16/2018

        RPS6KA1 plays a crucial role in stress responses in cancer cells, influencing cell survival and adaptation mechanisms.

        How does RPS6KA1 regulate protein synthesis and cell growth through the mTOR signaling pathway? 01/06/2018

        RPS6KA1 regulates protein synthesis and cell growth by modulating the mTOR pathway, affecting ribosomal protein activity.

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