| Description : |
MAP Kinase 2/Erk2 Protein is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. Involved in both the initiation and regulation of meiosis, mitosis, and post-mitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). |
| Source : |
E. coli |
| Species : |
Human |
| Tag : |
GST |
| Protein length : |
Full Length |
| Form : |
1.463mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 50% glycerol, 1mM benzamidine, 0.2mM PMSF, 0.1% 2- mercaptoethanol. Liquid at -20 centigrade. |
| Bio-activity : |
As provided, this lot demonstrated <1% of maximum activity. Activated by phosphorylation with MEK1(h). |
| Molecular Mass : |
67.8 kDa |
| AA Sequence : |
1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSMAAA AAAGAGPEMV 241 RGQVFDVGPR YTNLSYIGEG AYGMVCSAYD NVNKVRVAIK KISPFEHQTY CQRTLREIKI 301 LLRFRHENII GINDIIRAPT IEQMKDVYIV QDLMETDLYK LLKTQHLSND HICYFLYQIL 361 RGLKYIHSAN VLHRDLKPSN LLLNTTCDLK ICDFGLARVA DPDHDHTGFL TEYVATRWYR 421 APEIMLNSKG YTKSIDIWSV GCILAEMLSN RPIFPGKHYL DQLNHILGIL GSPSQEDLNC 481 IINLKARNYL LSLPHKNKVP WNRLFPNADS KALDLLDKML TFNPHKRIEV EQALAHPYLE 541 QYYDPSDEPI AEAPFKFDME LDDLPKEKLK ELIFEETARF QPGYRS |
| Purity : |
Purity 97.6% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -20 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
