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Recombinant Human MAPK1 cell lysate

Cat.No. : MAPK1-692HCL
Product Overview : Human MAPK1 / ERK2 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Baculovirus-Insect Cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : MAPK1 mitogen-activated protein kinase 1 [ Homo sapiens ]
Official Symbol : MAPK1
Synonyms : MAPK1; mitogen-activated protein kinase 1; PRKM1, PRKM2; ERK; ERK2; MAPK2; p41mapk; ERK-2; MAPK 2; p42-MAPK; MAP kinase 1; MAP kinase 2; MAP kinase isoform p42; protein tyrosine kinase ERK2; mitogen-activated protein kinase 2; extracellular signal-regulated kinase 2; p38; p40; p41; ERT1; PRKM1; PRKM2; P42MAPK;
Gene ID : 5594
mRNA Refseq : NM_002745
Protein Refseq : NP_002736
MIM : 176948
UniProt ID : P28482
Chromosome Location : 22q11.2
Pathway : ALK1 signaling events, organism-specific biosystem; ARMS-mediated activation, organism-specific biosystem; ATF-2 transcription factor network, organism-specific biosystem; Activated TLR4 signalling, organism-specific biosystem; Activation of NMDA receptor upon glutamate binding and postsynaptic events, organism-specific biosystem; Activation of the AP-1 family of transcription factors, organism-specific biosystem; Acute myeloid leukemia, organism-specific biosystem;
Function : ATP binding; DNA binding; MAP kinase activity; RNA polymerase II carboxy-terminal domain kinase activity; mitogen-activated protein kinase kinase kinase binding; nucleotide binding; phosphatase binding; phosphotyrosine binding; protein binding; protein serine/threonine kinase activity; transcription factor binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Can MAPK1 be used as a biomarker for cancer diagnosis? 12/28/2021

MAPK1 expression and activity levels can be used as biomarkers to assess cancer risk and prognosis.

How can MAPK1 be targeted in drug development for non-cancer conditions? 11/03/2021

MAPK1 inhibitors are being explored for potential use in non-cancer conditions, such as heart disease and neurodegenerative disorders.

In what other clinical conditions is MAPK1 relevant? 01/12/2020

MAPK1 is also implicated in various other diseases, including cardiovascular diseases and neurological disorders.

Are there targeted therapies that inhibit MAPK1 for cancer treatment? 10/27/2018

Yes, there are targeted therapies that aim to inhibit MAPK1, especially in cases where MAPK1 is overactive in cancer cells.

Can MAPK1 be used for personalized medicine approaches? 08/19/2017

Yes, by assessing the MAPK1 status in a patient's cells, healthcare providers can develop personalized treatment plans.

Customer Reviews (3)

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Reviews
03/24/2022

    Whether it be troubleshooting issues, offering experimental guidance, or addressing any concerns, the manufacturer's technical support ensures that researchers have a reliable and knowledgeable resource to rely on.

    03/26/2021

      In addition to its scientific merits, the manufacturer of MAPK1 protein stands out for its outstanding technical support.

      03/11/2020

        The company provides a dedicated team of experts who are readily available to assist researchers throughout their experimental journey.

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