Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953


  • Official Full Name

    MAPK3 mitogen-activated protein kinase 3

  • Synonyms

    MAPK3; mitogen-activated protein kinase 3; PRKM3; ERK1; p44erk1; p44mapk; MAPK 1; MAP kinase 1; MAP kinase 3; MAP kinase isoform p44; insulin-stimulated MAP2 kinase; mitogen-activated protein kinase 1; extracellular signal-related kinase 1; extracellular signal-regulated kinase 1; microtubule-associated protein 2 kinase; ERT2; ERK-1; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK; MGC20180;

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Protein Pre-coupled Magnetic Beads
  • Human
  • Mouse
  • Rat
  • Zebrafish
  • E. coli
  • E.coli
  • HEK293
  • HEK293T
  • Mammalian Cell
  • Mammalian cells
  • C
  • Myc
  • DDK
  • Flag
  • GST
  • His
  • His (Fc)
  • Avi
  • His|MBP
  • N/A
  • No tag
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human MAPK3-28699TH Recombinant Human MAPK3 E.coli N/A 378 amino acids
Human MAPK3-1031M Recombinant Human MAPK3 protein E.coli N/A Full Length
Human MAPK3-28H Recombinant human biotinylated ERK1, His-tagged E.coli His
Human MAPK3-34H Recombinant Human MAPK3 E.coli N/A
Human MAPK3-36H Recombinant Human MAPK3 E.coli N/A
Human MAPK3-1048H Recombinant Human Mitogen-Activated Protein Kinase 3 (Active), GST-Tagged E.coli GST
Human MAPK3-28694TH Recombinant Human MAPK3 E.coli N/A 379 amino acids
Human MAPK3-287H Recombinant Human MAPK3 protein, His/MBP-tagged E.coli His/MBP 146-210 aa
Human MAPK3-2787H Recombinant Human MAPK3, His-tagged E.coli His
Human MAPK3-1049H Recombinant Human Mitogen-Activated Protein Kinase 3 (Unactive), GST-Tagged E.coli GST
Human MAPK3-286H Recombinant Human MAPK3 protein, His/MBP-tagged E.coli His/MBP 246-360 aa
Human MAPK3-1517H Recombinant Human MAPK3 protein E.coli N/A
Human MAPK3-288H Recombinant Human MAPK3 protein, His/MBP-tagged E.coli His/MBP 12-84 aa
Human MAPK3-307H Active Recombinant Human MAPK3 Protein E.coli
Human MAPK3-4494HCL Recombinant Human MAPK3 293 Cell Lysate HEK293 N/A
Human ERK1-125H Recombinant Human MAPK3 protein, His-tagged E.coli His 238
Human MAPK3-061H Recombinant Human MAPK3 Protein, MYC/DDK-tagged, C13 and N15-labeled HEK293 C-Myc/DDK
Human MAPK3-1050H Active Recombinant Human MAPK3 Protein, His/GST-Tagged E. coli His&GST
Human MAPK3-2068HF Active Recombinant Full Length Human MAPK3 Protein, GST-tagged E.coli GST 1-379
Human MAPK3-1363H Recombinant Human MAPK3 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Human MAPK3-635HFL Recombinant Full Length Human MAPK3 Protein, C-Flag-tagged Mammalian cells Flag
Human MAPK3-1363H-B Recombinant Human MAPK3 Protein Pre-coupled Magnetic Beads HEK293
Human MAPK3-1082H Recombinant Human MAPK3 Protein (A2-P379), Tag Free E.coli No tag A2-P379
Human MAPK3-1083H Recombinant Human MAPK3 Protein (A2-P379), GST tagged E.coli GST A2-P379
Mouse MAPK3-9534M Recombinant Mouse MAPK3 Protein Mammalian Cell His
Mouse MAPK3-5350M-B Recombinant Mouse MAPK3 Protein Pre-coupled Magnetic Beads HEK293
Mouse MAPK3-5350M Recombinant Mouse MAPK3 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Mouse Mapk3-3942M Recombinant Mouse Mapk3 Protein, Myc/DDK-tagged HEK293T Myc/DDK
Rat MAPK3-3574R Recombinant Rat MAPK3 Protein Mammalian Cell His
Rat MAPK3-3230R Recombinant Rat MAPK3 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Rat MAPK3-3230R-B Recombinant Rat MAPK3 Protein Pre-coupled Magnetic Beads HEK293
Zebrafish MAPK3-11551Z Recombinant Zebrafish MAPK3 Mammalian Cell His
  • Background
  • Quality Guarantee
  • Case Study
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • MAPK3 Related Articles
  • MAPK3 Related Research Area
  • MAPK3 Related Signal Pathway

What is MAPK3 protein?

MAPK3 (mitogen-activated protein kinase 3) gene is a protein coding gene which situated on the short arm of chromosome 16 at locus 16p11. The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. The MAPK3 protein is consisted of 379 amino acids and its molecular mass is approximately 43.1 kDa.

What is the function of MAPK3 protein?

Activation of MAPK3 requires both tyrosine and threonine phosphorylation that is mediated by MEK. MAPK3 is ubiquitously distributed in tissues with the highest expression in heart, brain and spinal cord. Activated MAPK3 translocates into the nucleus where it phosphorylates various transcription factors (e.g., Elk-1, c-Myc, c-Jun, c-Fos, and C/EBP beta). MAPK3 can respond to extracellular stimuli, such as growth factors, hormones, and neurotransmitters, and activate downstream target proteins through phosphorylation. It is also involved in the regulation of immune response and can be activated by inflammatory mediators such as TNF-α and IL-1β, thus inducing the occurrence and maintenance of inflammatory response. It can also be activated by a variety of carcinogenic factors, such as Ras mutation, PTEN deletion, etc., and then promote the proliferation, invasion and metastasis of tumor cells.

MAPK3 Related Signaling Pathway

MAPK3 is an important member of the MAPK family, which can respond to a variety of extracellular stimuli, such as growth factors, hormones, neurotransmitters, and activate downstream target proteins through phosphorylation, thereby mediating cell proliferation, differentiation, apoptosis and other biological processes. MAPK3 is located between RAS GTPase and MAPK1 in the member kinase cascade, and MAPK3 can be activated by Ras proteins, which activate MAPK1 through phosphorylation, thereby regulating a variety of cell biological responses. In addition, MAPK3 can also be involved in the regulation of PI3K/AKT signaling pathway and Wnt/β-catenin signaling pathway.


Fig1. The proposed signaling pathways involved in Kisspeptin-modulated cell invasion and migration in endometrial cancer cells. (Hsien-Ming Wu, 2024)

MAPK3 Related Diseases

MAPK3 is abnormally expressed in a variety of cancers, including breast cancer, lung cancer, and colorectal cancer. It can promote the proliferation, invasion and metastasis of tumor cells by activating MAPK/ERK signaling pathway. MAPK3 can promote neuronal survival and axon growth by activating MAPK/ERK signaling pathway, thus participating in the occurrence and development of Parkinson's disease, Alzheimer's disease and other diseases. It can also promote inflammatory response and immune response by activating NF-κB signaling pathway, thus participating in the occurrence and development of autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus. In addition, the mutation of MAPK3 gene is associated with the occurrence of cardiovascular diseases such as hypertension and coronary heart disease.

Bioapplications of MAPK3

MAPK3 has important application value in many biological fields, and has important research significance for in-depth understanding of the mechanism of cell signaling, the mechanism of tumorigenesis, and the function and disease of nervous system and cardiovascular system. Therapeutic strategies targeting MAPK3 are also being investigated, such as the use of antibodies or small molecule inhibitors to inhibit its activity, thereby stopping tumor growth and metastasis.

High Purity



Fig2. Activity Data. (MAPK3-307H)

Case study 1: Jia Liu, 2016

Oleanolic acid (OA) is a natural triterpenoid that is widely distributed in edible and medicinal plants. OA exerts anti-tumor activity on a wide range of cancer cells primarily through inducing apoptosis. Dysregulated ERK signaling is closely complicated in the biology of cancer, such as metastasis, proliferation, and survival, and it can be activated by various stimuli. But it is not clear whether there is a connection between the OA and ERK signaling pathways.

In this study, by blocking ERK activation by U0126 or siRNAs was able to potentiate the pro-apoptotic activity of OA on cancer cells, the results showed that OA induced the activation of ERK in cancer cells. ERK activation compromised the apoptosis induced by OA. In conclusion, the researchers provided evidences that ERK activation is a mechanism underlying the resistance of cancer cells to OA-induced apoptosis and targeting ERK is a promising strategy to enhance the anti-tumor efficacy of OA.


Fig1. OA induces the activation of ERK signaling in cancer cells.

Fig2. A549 and PANC-1 cells were treated with OA (100 μg/mL) or/and U0126 (10 μM).

Case study 2: Yuanya Zhang, 2019

The spatiotemporal regulation of ERK activity has been extensively studied. However, scarce information has been available regarding the quality control of the kinases to scavenge malfunctioning ERKs.

Using site-specific mutagenesis and mass spectrometry, the researchers found that the disruption of the conserved H-bond between Y210 and E237 of ERK1 through point mutation at or naturally occurring nitration on Y210 initiates a quality control program dependent on chaperon systems and CHIP (C-terminal of Hsp70-interacting protein)-mediated ubiquitination and degradation. These findings clearly demonstrate how malfunctioning ERKs are eliminated when cells are in certain stress conditions or unhealthy states, and could represent a general mechanism for scavenging malfunctioning kinases in stress conditions.


Fig3. HEK293T cells expressing GFP-ERK1 (ERK1) or GFP-ERK1-Y210F (Y210F) were serum-starved overnight and then treated with the nuclear export inhibitor LMB (40 mM) or vehicle (DMSO) for 3 h as indicated.

Fig4. GFP fusion of ERK1 or Y210F in serum-starved or MG132-treated (5 μM) HEK293 cells was IPed with anti-GFP antibody and probed for Hsp90 by Western blotting.

Fig1. The diagramed model shows Y210 modification-induced ERK1 ubiquitination and degradation. (Yuanya Zhang, 2019)


Fig2. BMPs signal via the canonical, Smaddependent pathway or various non-canonical pathways. (Mohamad Z Mehdi, 2007)

MAPK3 involved in several pathways and played different roles in them. We selected most pathways MAPK3 participated on our site, such as MAPK signaling pathway, ErbB signaling pathway, Ras signaling pathway, which may be useful for your reference. Also, other proteins which involved in the same pathway with MAPK3 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
cGMP-PKG signaling pathwayATP1A1;IRS4;BNP;ROCK2;Adcy4;PIK3CA;CALML3;GTF2I;MAPK1
cAMP signaling pathwayEP300;PPP1CA;PPP1CC;Adcy4;FXYD1;MAPK1;ARAP3;PIK3R2;NPY1R
Chemokine signaling pathwayGNAI3;GM13304;CCR7;GNG3;LYN;CXCR6;VAV2;CXCL3;PTK2
HIF- signaling pathwayPLCG1;INS1;AKT2;PDHA1;CAMK2D;NFKB1;HK3;CAMK2B;PGK1
Sphingolipid signaling pathwayPPP2R2B;PLD2;PLCB4;CERS2;SPTLC1;PRKCG;DEGS2;SGPP2;PLCB2
Phospholipase D signaling pathwayMAP2K1;PIP5K1C;KRAS;CXCR1;SHC2;PLD1;PIK3R5;AGTR1B;GRM1
Adrenergic signaling in cardiomyocytesPPP2R2D;CACNB3A;PPP2R3A;CALML3;ATP1A3B;CACNG8;ACTC1B;MYL3;CACNA1D
Vascular smooth muscle contractionMAP2K2B;ADCY1A;PLCB3;PRKCH;RHOA;MYL6;PLA2G4F;ITPR3;GNAS
Dorso-ventral axis formationSPIRE2;MAPK1;GRB2A;GRB2;ETS1;PPP1R2P9;PIWIL1;NOTCH1B;FMN2
TGF-beta signaling pathwayTFDP1A;ID1;TGFBR2;BMP6;CHRD;RHOAC;ID3;ID4;LTBP1
Signaling pathways regulating pluripotency of stem cellsSMAD9;BMPR1B;ACVR1C;ESX1;ZIC3;AXIN2;Fzd4;BMPR1A;ISL1
Platelet activationGUCY1B3;RHOA;ARHGAP35;MYLK2;Adcy4;MAPK3;MAPK11;TBXAS1;MYL12A
Toll-like receptor signaling pathwayLBP;IL12BA;MAP2K6;IRF3;CXCL11;MYD88;TLR3;CXCL9;PIK3CA
NOD-like receptor signaling pathwaySUGT1;NLRC4;MAP3K7;MAP3K7IP3;MAPK12A;IKBKB;IL6;NFKBIB;RELA
Natural killer cell mediated cytotoxicityHCST;RAET1G;Raet1a;PRKCA;IFNA10;IFNA16;KIR2DS5;BRAF;ITGB2L
T cell receptor signaling pathwayIFNG;LCP2;PIK3CD;LCK;FYN;MAP3K7;IL5;CBLB;AKT1
B cell receptor signaling pathwayCRKL;SOS1;MAP4K1;ARPC4;cgr2b;BCL10;HDAC5;NFATC2;CD79B
Fc epsilon RI signaling pathwayMAPK11;MAP2K7;MAPK9;PIK3R1;PLA2G4F;KRAS;HRAS;LAT;IL13
Fc gamma R-mediated phagocytosisPIK3R2;FCGR2B;PRKCA;MARCKS;GSN;PIP5K1B;ASAP1;SPHK1;ARPC5L
TNF signaling pathwayIL1B;LIF;MAPK8;MAPK11;TAB2;LTA;AKT2;Ccl12;CXCL2
Neurotrophin signaling pathwayCALML5;RPS6KA1;RELA;ARHGDIA;MAP2K1;MAGED1;CDC42;KIDINS220;MAPK1
Retrograde endocannabinoid signalingGNGT2;MAPK1;SLC17A8;GNG10;PLCB1;MAPK10;GNAI2;GABRD;GNAO1
Cholinergic synapseCHRM5;GNG10;KCNQ4;GNAI3;PIK3CA;KCNJ6;GNAI1;KCNJ12;JAK2
Serotonergic synapseGNAS;ITPR1;HTR5A;GNG12;CYP2C50;PLA2G4C;PLA2G4D;CYP2C55;PTGS1
Long-term depressionITPR1;MAPK3;GNAI2;GNA11;GUCY1B3;PLCB4;ITPR3;KRAS;IGF1R
Regulation of actin cytoskeletonARHGEF12;ITGA2;WASB;PIP4K2C;FGD;RAC3;MAP2K2B;MAP2K2A;RHOAA
Insulin signaling pathwayAKT2L;BAD;FBP1B;SOCS2;HK2;CALML5;PRKAR1AB;PHKG1A;LIPE
Progesterone-mediated oocyte maturationANAPC13;MAPK13;MAPK9;PIK3CG;PIK3R1;MAPK10;FZR1A;INS1;RPS6KA2
Estrogen signaling pathwayAKT1;FOS;CALML3;CALM4;PLCB1;PIK3CG;HSPA1L;HSPA1B;MMP2
Prolactin signaling pathwaySOCS7;PIK3CB;NRAS;SIRPA;SOCS2;CSN2;AKT2;CCND2;FOXO3
Thyroid hormone signaling pathwayNOTCH3;RAF1;HDAC3;PRKACB;PLCD1;TRP53;ATP1A3;PLN;ESR1
Oxytocin signaling pathwayPRKAA2;MYLK;PRKACB;RAF1;NPR1;CALM2;PLCB2;GUCY1B3;HRAS
Type II diabetes mellitusPIK3R3;TNF;MAPK3;PIK3CG;HK1;PKLR;PRKCD;IRS4;SLC2A4
Aldosterone-regulated sodium reabsorptionINS2;MAPK3;ATP1A3;INSR;INS1;IRS1;PIK3R1;SCNN1G;FXYD4
Prion diseasesELK1;HSPA1A;C9;IL6;C6;NCAM2;CCL5;IL1B;PRKACB
Salmonella infectionIL-8;TJP1;MAPK14B;PLEKHM2;WASLA;IL6;ROCK2A;RAB7B;LBP
Chagas disease (American trypanosomiasis)CCL3L1;CD3D;IKBKB;GNAI3;GNAS;GNAQ;C1QC;MAPK9;Ccl12
Colorectal cancerTP53;RAF1;LEF1;BRAF;CCND1;MAP2K1;APC2;PIK3R2;APC
Renal cell carcinomaRAF1;MAP2K1;MAPK1;SLC2A1;HRAS;ARNT;CUL2;PAK3;PAK2
Chronic myeloid leukemiaCBLC;AKT1;MAPK3;TGFB2;MECOM;TGFB1;SOS1;PIK3R5;CRK
Non-small cell lung cancerCCND1;ERBB2;PIK3R5;GRB2;PIK3CA;AKT1;PRKCA;RXRB;PIK3CB
Central carbon metabolism in cancerMAP2K1;IDH1;PFKM;SLC2A2;PIK3CD;G6PDX;PKM;AKT2;PIK3CG
Choline metabolism in cancerNRAS;DGKD;LYPLA1;WASF2;PDGFRA;PIP5K1A;MAPK1;PPAP2A;PIK3R2

MAPK3 has several biochemical functions, for example, ATP binding, MAP kinase activity, phosphatase binding. Some of the functions are cooperated with other proteins, some of the functions could acted by MAPK3 itself. We selected most functions MAPK3 had, and list some proteins which have the same functions with MAPK3. You can find most of the proteins on our site.

Function Related Protein
phosphatase bindingDLG3;CHCHD3;ELFN2;MAGI2;TPRN;SH3RF2;KLRAQ1;SMAD3;PPP1R21
phosphotyrosine bindingSAMSN1;SHC1;ACP2;MAPK3;CBLC;FGR;CBL;PTPN3;ZAP70
scaffold protein bindingSCN5A;KCNH2;NLGN1;NLGN4Y;PANX1;NLRP1;P2RY1;CACNA1H;TREM1

MAPK3 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with MAPK3 here. Most of them are supplied by our site. Hope this information will be useful for your research of MAPK3.


Blaha, M; Nemcova, L; et al. Cyclic guanosine monophosphate does not inhibit gonadotropin-induced activation of mitogen-activated protein kinase 3/1 in pig cumulus-oocyte complexes. REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY 13:-(2015).
Chen, TC; Lin, KT; et al. Using an in Situ Proximity Ligation Assay to Systematically Profile Endogenous Protein-Protein Interactions in a Pathway Network. JOURNAL OF PROTEOME RESEARCH 13:5339-5346(2014).
  • Q&As
  • Reviews

Q&As (7)

Ask a question
How is MAPK3 involved in disease development? 04/29/2023

Dysregulation of MAPK3 signaling has been implicated in the pathogenesis of several diseases. Aberrant activation of MAPK3 is associated with cancer development and progression, as it promotes cell proliferation and survival. MAPK3 signaling is also involved in cardiovascular diseases, contributing to vascular remodeling and hypertrophy. In neurodegenerative diseases, dysregulated MAPK3 activity contributes to neuronal dysfunction and apoptosis.

What are the future directions in MAPK3 research? 03/08/2023

Future research in MAPK3 aims to elucidate the intricate signaling network and identify novel regulatory mechanisms. Understanding the crosstalk between MAPK3 and other signaling pathways will be crucial for developing targeted therapies. Additionally, exploring the role of MAPK3 isoforms in different cellular contexts and disease states will provide valuable insights. Furthermore, the development of more specific and potent inhibitors with reduced toxicity profiles is a focus for future therapeutic interventions.

 What is the molecular structure and function of MAPK3 protein? 09/24/2022

MAPK3, also known as ERK1 (Extracellular Signal-Regulated Kinase 1), is a serine/threonine kinase involved in cell signaling pathways. It consists of 379 amino acids and exhibits a conserved catalytic domain. MAPK3 plays a crucial role in regulating cell proliferation, differentiation, and survival by phosphorylating downstream targets such as transcription factors and other kinases.

What are the downstream effectors of MAPK3 signaling? 11/08/2021

MAPK3 signaling exerts its effects through phosphorylation of downstream targets. It phosphorylates various transcription factors, including ELK1 and c-Fos, leading to changes in gene expression. MAPK3 also phosphorylates other kinases, such as p90RSK, which further propagate the signal to regulate cellular processes like cell cycle progression, apoptosis, and differentiation.

What are the current challenges in targeting MAPK3 for therapy? 01/06/2020

One challenge is the potential for off-target effects when targeting MAPK3, as it is involved in multiple signaling pathways. Additionally, resistance to MAPK3 inhibitors can emerge due to compensatory signaling mechanisms and genetic alterations. Another challenge lies in achieving selective inhibition of specific MAPK3 isoforms without affecting other closely related kinases.

How is the activity of MAPK3 regulated? 11/16/2019

The activity of MAPK3 is tightly regulated by various mechanisms. Activation of MAPK3 involves dual phosphorylation of threonine and tyrosine residues by upstream kinases. Conversely, dephosphorylation by phosphatases can inactivate MAPK3. Additionally, scaffolding proteins and interacting partners can modulate its activity by facilitating or inhibiting its phosphorylation or by regulating its subcellular localization.

What therapeutic strategies target MAPK3? 02/20/2019

Inhibition of MAPK3 activity or downstream effectors represents a potential therapeutic approach. Small molecule inhibitors targeting MAPK3 kinase activity, such as U0126 and PD98059, have been developed. Monoclonal antibodies against MAPK3 or its downstream effectors are also being investigated. Combination therapies targeting multiple components of the MAPK3 signaling pathway are being explored to enhance efficacy and overcome resistance.

Customer Reviews (3)

Write a review

    Exemplary precision and unblemished dependability render it my most cherished research ally.


      Presenting exceptional reliability and stability, the experimental reagent establishes a robust foundation for my experiments.


        Exquisitely crafted packaging design adds convenience to the usage of this protein reagent and exudes a sense of sophistication.

        Ask a Question for All MAPK3 Products

        Required fields are marked with *

        My Review for All MAPK3 Products

        Required fields are marked with *


        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends