| Description : |
Eph (erythropoietin-producing hepatocellular) family tyrosine kinase receptors consist of two groups: EphA group members respond to ephrin-A, which is anchored to the cell membrane by glycosylphosphatidylinositol, whereas EphB group members respond to ephrin-B, containing a transmembrane domain. High levels of EphA2 have been documented in many cell models and clinical specimens of human cancer, including metastatic melanoma and breast, prostate, lung, renal cell, colon, and esophageal carcinomas. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
596-900 aa |
| Form : |
1.18mg/ml of enzyme in 50mM HEPES pH7.0, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
268U/mg, where one unit of EphA2 activity is defined as 1nmol phosphate incorporated into 0.1mg/ml poly(Glu, Tyr) (4:1) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
38 kDa |
| AA Sequence : |
1 MSYYHHHHHH DYDIPTTENL YFQGAMGSDP NQAVLKFTTE IHPSCVTRQK VIGAGEFGEV 61 YKGMLKTSSG KKEVPVAIKT LKAGYTEKQR VDFLGEAGIM GQFSHHNIIR LEGVISKYKP 121 MMIITEYMEN GALDKFLREK DGEFSVLQLV GMLRGIAAGM KYLANMNYVH RDLAARNILV 181 NSNLVCKVSD FGLSRVLEDD PEATYTTSGG KIPIRWTAPE AISYRKFTSA SDVWSFGIVM 241 WEVMTYGERP YWELSNHEVM KAINDGFRLP TPMDCPSAIY QLMMQCWQQE RARRPKFADI 301 VSILDKLIRA PDSLKTLADF DPRVSIRLPS TSG |
| Purity : |
Purity 96% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
