Recombinant Human EPHA2 cell lysate
| Cat.No. : | EPHA2-001HCL |
| Product Overview : | Human EPHA2 (aa 585-976) derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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| Source : | Baculovirus-Insect Cells |
| Species : | Human |
| Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
| Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
| Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
| Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
| Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
| Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
| Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
| Gene Name : | EPHA2 EPH receptor A2 [ Homo sapiens ] |
| Official Symbol : | EPHA2 |
| Synonyms : | EPHA2; EPH receptor A2; ECK, EphA2; ephrin type-A receptor 2; soluble EPHA2 variant 1; tyrosine-protein kinase receptor ECK; epithelial cell receptor protein tyrosine kinase; ECK; CTPA; ARCC2; CTPP1; |
| Gene ID : | 1969 |
| mRNA Refseq : | NM_004431 |
| Protein Refseq : | NP_004422 |
| MIM : | 176946 |
| UniProt ID : | P29317 |
| Chromosome Location : | 1p36 |
| Pathway : | Arf6 signaling events, organism-specific biosystem; Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Direct p53 effectors, organism-specific biosystem; EPHA forward signaling, organism-specific biosystem; EPHA2 forward signaling, organism-specific biosystem; EphrinA-EPHA pathway, organism-specific biosystem; |
| Function : | ATP binding; ephrin receptor activity; nucleotide binding; protein binding; receptor activity; transmembrane receptor protein tyrosine kinase activity; |
Products Types
| ◆ Recombinant Protein | ||
| EPHA2-321H | Recombinant Human EPHA2 Protein, MYC/DDK-tagged, C13 and N15-labeled | +Inquiry |
| Epha2-954M | Recombinant Mouse Epha2 Protein, MYC/DDK-tagged | +Inquiry |
| Epha2-38M | Recombinant Mouse Epha2 Protein, His (Fc)-Avi-tagged | +Inquiry |
| EPHA2-1903H | Recombinant Human EPHA2 Protein, MYC/DDK-tagged | +Inquiry |
| EPHA2-0985H | Recombinant Human EPHA2 Protein (R561-I976), Tag Free | +Inquiry |
| ◆ Lysates | ||
| EPHA2-1072HCL | Recombinant Human EPHA2 cell lysate | +Inquiry |
| EPHA2-2138MCL | Recombinant Mouse EPHA2 cell lysate | +Inquiry |
| ◆ Assay kits | ||
| Kit-0326 | EphA2 Kinase (Human) Assay/Inhibitor Screening Assay Kit | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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