Active Recombinant Human RPS6KA3(2-end) Protein, N-His-tagged

• Cat.No.: RPS6KA3-221H
• Product Overview: N-terminal 6His-tagged recombinant human RSK2 residues 2–end. Expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA-agarose. Activated with MAPK2 and PDK1, and re-purified by glutathione, heparin and Ni2+/NTA-agarose chromatographies.

Specification

• Description: Rsk2, also known as Ribosomal Protein S6 Kinase A3, is a member of the RSK family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) Signaling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS). The protein sequence is identical to that described in GenBank NM_004586 for human RSK2, over residues 2–end.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 2-end
• Form: 0.39mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2- mercaptoethanol.
• Bio-activity: 5067U/mg, where one unit of RSK2, active activity is defined as 1nmol phosphate incorporated into 30μM (KKKNRTLSVA) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 85 kDa
• AA Sequence: 1 MAHHHHHHGS PLAQLADPWQ KMAVESPSDS AENGQQIMDE PMGEEEINPQ TEEVSIKEIA 61 ITHHVKEGHE KADPSQFELL KVLGQGSFGK VFLVKKISGS DARQLYAMKV LKKATLKVRD 121 RVRTKMERDI LVEVNHPFIV KLHYAFQTEG KLYLILDFLR GGDLFTRLSK EVMFTEEDVK 181 FYLAELALAL DHLHSLGIIY RDLKPENILL DEEGHIKLTD FGLSKESIDH EKKAYSFCGT 241 VEYMAPEVVN RRGHTQSADW WSFGVLMFEM LTGTLPFQGK DRKETMTMIL KAKLGMPQFL 301 SPEAQSLLRM LFKRNPANRL GAGPDGVEEI KRHSFFSTID WNKLYRREIH PPFKPATGRP 361 EDTFYFDPEF TAKTPKDSPG IPPSANAHQL FRGFSFVAIT SDDESQAMQT VGVHSIVQQL 421 HRNSIQFTDG YEVKEDIGVG SYSVCKRCIH KATNMEFAVK IIDKSKRDPT EEIEILLRYG 481 QHPNIITLKD VYDDGKYVYV VTELMKGGEL LDKILRQKFF SEREASAVLF TITKTVEYLH 541 AQGVVHRDLK PSNILYVDES GNPESIRICD FGFAKQLRAE NGLLMTPCYT ANFVAPEVLK 601 RQGYDAACDI WSLGVLLYTM LTGYTPFANG PDDTPEEILA RIGSGKFSLS GGYWNSVSDT 661 AKDLVSKMLH VDPHQRLTAA LVLRHPWIVH WDQLPQYQLN RQDAPHLVKG AMAATYSALN 721 RNQSPVLEPV GRSTLAQRRG IKKITSTAL
• Purity: Purity 77% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: RPS6KA3 ribosomal protein S6 kinase A3 [ Homo sapiens (human) ]
• Official Symbol: RPS6KA3
• Synonyms: RPS6KA3; ribosomal protein S6 kinase A3; CLS; RSK; HU-3; RSK2; MRX19; ISPK-1; XLID19; p90-RSK2; pp90RSK2; MAPKAPK1B; S6K-alpha3
• Gene ID: 6197
• mRNA Refseq: NM_004586.3
• Protein Refseq: NP_004577.1
• MIM: 300075
• UniProt ID: P51812