Description : |
MSK1 (mitogen- and stress-activated protein kinases-1, ribosomal protein S6 kinase, 90kDa, polypeptide 5) and MSK2 (mitogen- and stress-activated protein kinases-2, ribosomal protein S6 kinase, 90kDa, polypeptide 4) are activated in vitro by MAPK2/ERK2 or SAPK2/p38. Endogenous MSK is activated by UV radiation, oxidative stress, and other cell-damaging stimuli. MSK may mediate the growth factor and stress-induced activation of CREB. |
Source : |
Sf21 |
Species : |
Human |
Tag : |
Flag,His |
Protein length : |
2-end |
Form : |
2.222mg/ml of enzyme in 50mM Tris/HCl pH7.5, 50% glycerol, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF, 0.1 % 2- mercaptoethanol. Frozen solution. |
Bio-activity : |
734U/mg, where one unit of MSK1, active activity is defined as 1nmol phosphate incorporated into 30μM modified crosstide (GRPRTSSFAEGKK) per minute at 30 centigrade with a final ATP concentration of 100μM. |
Molecular Mass : |
94.4 kDa |
AA Sequence : |
1 MSYYHHHHHH DYDIPTTENL YFQGAMGSAT MDYKDDDDKE EEGGSSGGAA GTSADGGDGG 61 EQLLTVKHEL RTANLTGHAE KVGIENFELL KVLGTGAYGK VFLVRKISGH DTGKLYAMKV 121 LKKATIVQKA KTTEHTRTER QVLEHIRQSP FLVTLHYAFQ TETKLHLILD YINGGELFTH 181 LSQRERFTEH EVQIYVGEIV LALEHLHKLG IIYRDIKLEN ILLDSNGHVV LTDFGLSKEF 241 VADETERAYS FCGTIEYMAP DIVRGGDSGH DKAVDWWSLG VLMYELLTGA SPFTVDGEKN 301 SQAEISRRIL KSEPPYPQEM SALAKDLIQR LLMKDPKKRL GCGPRDADEI KEHLFFQKIN 361 WDDLAAKKVP APFKPVIRDE LDVSNFAEEF TEMDPTYSPA ALPQSSEKLF QGYSFVAPSI 421 LFKRNAAVID PLQFHMGVER PGVTNVARSA MMKDSPFYQH YDLDLKDKPL GEGSFSICRK 481 CVHKKSNQAF AVKIISKRME ANTQKEITAL KLCEGHPNIV KLHEVFHDQL HTFLVMELLN 541 GGELFERIKK KKHFSETEAS YIMRKLVSAV SHMHDVGVVH RDLKPENLLF TDENDNLEIK 601 IIDFGFARLK PPDNQPLKTP CFTLHYAAPE LLNQNGYDES CDLWSLGVIL YTMLSGQVPF 661 QSHDRSLTCT SAVEIMKKIK KGDFSFEGEA WKNVSQEAKD LIQGLLTVDP NKRLKMSGLR 721 YNEWLQDGSQ LSSNPLMTPD ILGSSGAAVH TCVKATFHAF NKYKREGFCL QNVDKAPLAK 781 RRKMKKTSTS TETRSSSSES SHSSSSHSHG KTTPTKTLQP SNPADSNNPE TLFQFSDSVA |
Purity : |
Purity 93.5% by SDS-PAGE and Coomassie blue staining. |
Applications : |
Drug Discovery & Development |
Storage : |
1 year at -70 centigrade. |
Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
Use/Stability : |
On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |