Active Recombinant Human RPS6KA5(2-end) Protein, N-His-tagged

• Cat.No.: RPS6KA5-160H
• Product Overview: N-terminal 6His-tagged recombinant human MSK1 residues 2–end. Expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose, activated using human MAPK2, and then re-purified using Ni2+/NTA agarose.

Specification

• Description: Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidermal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-Jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 2-end
• Form: 2.043mg/ml of enzyme in 50mM Tris/HCl pH7.5, 50% glycerol, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Liquid at -20 centigrade.
• Bio-activity: 404U/mg, where one unit of MSK1, active activity is defined as 1nmol phosphate incorporated into 30μM modified crosstide (GRPRTSSFAEGKK) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 94 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF KEEEGGSSGG AAGTSADGGD GGEQLLTVKH 61 ELRTANLTGH AEKVGIENFE LLKVLGTGAY GKVFLVRKIS GHDTGKLYAM KVLKKATIVQ 121 KAKTTEHTRT ERQVLEHIRQ SPFLVTLHYA FQTETKLHLI LDYINGGELF THLSQRERFT 181 EHEVQIYVGE IVLALEHLHK LGIIYRDIKL ENILLDSNGH VVLTDFGLSK EFVADETERA 241 YSFCGTIEYM APDIVRGGDS GHDKAVDWWS LGVLMYELLT GASPFTVDGE KNSQAEISRR 301 ILKSEPPYPQ EMSALAKDLI QRLLMKDPKK RLGCGPRDAD EIKEHLFFQK INWDDLAAKK 361 VPAPFKPVIR DELDVSNFAE EFTEMDPTYS PAALPQSSEK LFQGYSFVAP SILFKRNAAV 421 IDPLQFHMGV ERPGVTNVAR SAMMKDSPFY QHYDLDLKDK PLGEGSFSIC RKCVHKKSNQ 481 AFAVKIISKR MEANTQKEIT ALKLCEGHPN IVKLHEVFHD QLHTFLVMEL LNGGELFERI 541 KKKKHFSETE ASYIMRKLVS AVSHMHDVGV VHRDLKPENL LFTDENDNLE IKIIDFGFAR 601 LKPPDNQPLK TPCFTLHYAA PELLNQNGYD ESCDLWSLGV ILYTMLSGQV PFQSHDRSLT 661 CTSAVEIMKK IKKGDFSFEG EAWKNVSQEA KDLIQGLLTV DPNKRLKMSG LRYNEWLQDG 721 SQLSSNPLMT PDILGSSGAA VHTCVKATFH AFNKYKREGF CLQNVDKAPL AKRRKMKKTS 781 TSTETRSSSS ESSHSSSSHS HGKTTPTKTL QPSNPADSNN PETLFQFSDS VA
• Purity: Purity 93.3% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: RPS6KA5 ribosomal protein S6 kinase A5 [ Homo sapiens (human) ]
• Official Symbol: RPS6KA5
• Synonyms: RPS6KA5; ribosomal protein S6 kinase A5; MSK1; RLPK; MSPK1
• Gene ID: 9252
• mRNA Refseq: NM_001322227.2
• Protein Refseq: NP_001309156.1
• MIM: 603607
• UniProt ID: O75582