Active Recombinant Human RPS6KB1(1-421) Protein, N-His-tagged

• Cat.No.: RPS6KB1-177H
• Product Overview: N-terminal 6Histagged, recombinant amino acids 1–421 of human p70 S6 kinase expressed in Sf21 insect cells. Purified using Ni2+/NTAagarose, activated by PDK1, and repurified on heparin-agarose.

Specification

• Description: p70 S6 Kinase, also known as RPS6KB1 (Ribosomal Protein S6 Kinase B1) is a Protein Coding gene. This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. GenBank M60724. The recombinant protein contains an amino acid substitution, T412E, with reference to M60724. This mutation mimics phosphorylation of T412 and facilitates activation by PDK1.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 1-421 aa
• Form: 1.032mg/ml of enzyme in 50mM Tris/HCl pH7.5, 270mM sucrose, 150mM NaCl, 1mM benzamidine, 0.2mM PMSF, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij-35. Frozen solution.
• Bio-activity: 280U/mg, where one unit of p70 S6 kinase, active activity is defined as 1nmol phosphate incorporated into 100μM (KKRNRTLTV) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 48.7 kDa
• AA Sequence: 1 MHHHHHHMRR RRRRDGFYPA PDFRDREAED MAGVFDIDLD QPEDAGSEDE LEEGGQLNES 61 MDHGGVGPYE LGMEHCEKFE ISETSVNRGP EKIRPECFEL LRVLGKGGYG KVFQVRKVTG 121 ANTGKIFAMK VLKKAMIVRN AKDTAHTKAE RNILEEVKHP FIVDLIYAFQ TGGKLYLILE 181 YLSGGELFMQ LEREGIFMED TACFYLAEIS MALGHLHQKG IIYRDLKPEN IMLNHQGHVK 241 LTDFGLCKES IHDGTVTHTF CGTIEYMAPE ILMRSGHNRA VDWWSLGALM YDMLTGAPPF 301 TGENRKKTID KILKCKLNLP PYLTQEARDL LKKLLKRNAA SRLGAGPGDA GEVQAHPFFR 361 HINWEELLAR KVEPPFKPLL QSEEDVSQFD SKFTRQTPVD SPDDSTLSES ANQVFLGFEY 421 VAPSVLES
• Purity: Purity 89.8% by SDSPAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: RPS6KB1 ribosomal protein S6 kinase B1 [ Homo sapiens (human) ]
• Official Symbol: RPS6KB1
• Synonyms: RPS6KB1; ribosomal protein S6 kinase B1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha
• Gene ID: 6198
• mRNA Refseq: NM_001272042.2
• Protein Refseq: NP_001258971.1
• MIM: 608938
• UniProt ID: P23443