||Recombinant human UBE2N protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
||UBE2N, also known as UBC13, is a member of the E2 ubiquitin-conjugating enzyme family. It catalyzes the ATP-dependent synthesis of non-canonical polyubiquitin chains, a process that does not lead to proteasomal degradation. It mediates the transcription of several target genes and is thought to play a role in cell cycle progression; cellular differentiation and DNA repair mechanisms that ensure cell survival after DNA damage.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT
||19.3 kDa(172aa), confirmed by MALDI-TOF
||> 95% by SDS - PAGE
||0.5 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MAGLPRRIIK ETQRLLAEPV PGIKAEPDES NARYFHVVIA GPQDSPFEGG TFKLELFLPE EYPMAAPKVRFMTKIYHPNV DKLGRICLDI LKDKWSPALQ IRTVLLSIQA LLSAPNPDDP LANDVAEQWK TNEAQAIETA RAWTRLYAMN NI
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.