Recombinant Full Length Human PPP1CA Protein

• Cat.No.: PPP1CA-159HFL
• Product Overview: Untagged, recombinant, full length, human PP1 alpha expressed in E.coli cells. Purified using Heparin sepharose followed by Q sepharose.

Specification

• Description: PP1α protein is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Source: E. coli
• Species: Human
• Protein length: Full Length
• Form: 0.63mg/ml of enzyme in 50mM Hepes/NaOH pH7.5, 300mM NaCl, 50% glycerol, 0.1mM EGTA, 1mM MnCl2, 0.03% Brij-35, 0.1mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Liquid at -20 centigrade.
• Bio-activity: 12373Units/mg. A Unit releases 1nmole of phosphate min-1 from the phosphorylated substrate DiFMUP.
• Molecular Mass: 37.6 kDa
• AA Sequence: 1 MSDSEKLNLD SIIGRLLEVQ GSRPGKNVQL TENEIRGLCL KSREIFLSQP ILLELEAPLK 61 ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS LETICLLLAY KIKYPENFFL 121 LRGNHECASI NRIYGFYDEC KRRYNIKLWK TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL 181 QSMEQIRRIM RPTDVPDQGL LCDLLWSDPD KDVQGWGEND RGVSFTFGAE VVAKFLHKHD 241 LDLICRAHQV VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAD 301 KNKGKYGQFS GLNPGGRPIT PPRNSAKAKK
• Purity: Purity 59% by SDS PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PPP1CA protein phosphatase 1 catalytic subunit alpha [ Homo sapiens (human) ]
• Official Symbol: PPP1CA
• Synonyms: PPP1CA; protein phosphatase 1 catalytic subunit alpha; PP1A; PP-1A; PPP1A; PP1alpha
• Gene ID: 5499
• mRNA Refseq: NM_206873.2
• Protein Refseq: NP_996756.1
• MIM: 176875
• UniProt ID: P62136