||Recombinant mouse S100A9 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography (1-113aa).
||S100A9, also known as Calgranulin-B, belongs to the S100 family containing EF-hand type Ca2+-binding proteins. It is involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation, and associated with the disease cystic fibrosis. S100A9 has been implicated in the abnormal differentiation of myeloid cells in the stroma of cancer. This protein may function in the inhibition of casein kinase.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT
||15.2 kDa (133aa), confirmed by MALDI-TOF
||> 90% by SDS - PAGE
||0.5 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MANKAPSQME RSITTIIDTF HQYSRKEGHP DTLSKKEFRQ MVEAQLATFM KKEKRNEALI NDIMEDLDTN QDNQLSFEEC MMLMAKLIFA CHEKLHENNP RGHGHSHGKG CGK
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°Cor -70°C. Avoid repeated freezing and thawing cycles.