S100A4

  • Official Full Name

    S100 calcium binding protein A4

  • Overview

    The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
  • Synonyms

    S100A4; S100 calcium binding protein A4; 42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98; protein S100-A4; protein Mts1; fibroblast-specific protein-1; placental calcium-binding protein; malignant transformation suppression 1; leukemia multidrug resistance associated protein; S100 calcium-binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog);

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Protein Pre-coupled Magnetic Beads
  • Cattle
  • Human
  • Mouse
  • Rat
  • E.coli
  • HEK293
  • HEK293T
  • Human Cell
  • Mammalian Cell
  • Mammalian cells
  • Mouse
  • C
  • Myc
  • DDK
  • Flag
  • GST
  • hFc
  • His
  • His (Fc)
  • Avi
  • His|Myc
  • SUMO
  • N/A
  • N
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human S100A4-201H Recombinant Human S100 Calcium Binding Protein A4 E.coli N/A
Human S100A4-70H Recombinant Human S100A4 protein, hFc-tagged HEK293 hFc Met1-Lys101
Human S100A4-89H Recombinant Human S100A4 E.coli N/A
Human S100A4-409H Recombinant Human S100 Calcium Bbinding Protein A4, His-tagged E.coli His
Human S100A4-3727H Recombinant Human S100A4, His-tagged E.coli His
Human S100A4-2489H Recombinant Human S100A4, GST-tagged E.coli GST 1-101aa
Human S100A4-31367TH Recombinant Human S100A4 E.coli N/A
Human S100A4-162H Recombinant Human S100A4 Mammalian Cell His
Human S100A4-195H Recombinant Human S100A4 protein, His-tagged E.coli His Met1-Lys101
Human S100A4-2859HCL Recombinant Human S100A4 cell lysate Human Cell N/A
Human S100A4-3461H Recombinant Human S100A4 protein, His-SUMO-tagged E.coli His-SUMO 2-101aa
Human S100A4-4590H Recombinant Human S100A4 Protein, Myc/DDK-tagged, C13 and N15-labeled HEK293T Myc/DDK
Human S100A4-385H Recombinant Human S100A4 Protein, MYC/DDK-tagged, C13 and N15-labeled HEK293 C-Myc/DDK
Human S100A4-1940H-B Recombinant Human S100A4 Protein Pre-coupled Magnetic Beads HEK293
Human S100A4-1051HFL Recombinant Full Length Human S100A4 Protein, C-Flag-tagged Mammalian cells Flag
Human S100A4-6215H Recombinant Human S100A4 Protein (Met1-Lys101), N-His tagged E.coli N-His Met1-Lys101
Human S100A4-1940H Recombinant Human S100A4 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Mouse S100A4-159M Recombinant Mouse S100a4, Fc tagged Human Cell His
Mouse S100a4-532M Recombinant Mouse S100a4 Human Cell N/A Ala2-Lys101
Mouse S100a4-3542M Recombinant Mouse S100a4, His-tagged E.coli His
Mouse S100a4-533M Recombinant Mouse S100a4 protein, His-tagged E.coli His Met1-Lys101
Mouse S100a4-5105M Recombinant Mouse S100 calcium Binding Protein A4, His-tagged Mouse His
Mouse S100A4-001MCL Recombinant Mouse S100A4 cell lysate Human Cell N/A
Mouse S100a4-5669M Recombinant Mouse S100a4 Protein, Myc/DDK-tagged HEK293T Myc/DDK
Mouse S100a4-566M Recombinant Mouse S100a4 Protein, hFc-tagged HEK293 hFc Ala2-Lys101
Mouse S100a4-7323M Recombinant Mouse S100a4 Protein, His-tagged E.coli His 1-101
Rat S100A4-5213R Recombinant Rat S100A4 Protein Mammalian Cell His
Rat S100A4-4872R Recombinant Rat S100A4 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Rat S100A4-2599R Recombinant Rat S100A4 Protein (2-101 aa), His-Myc-tagged E.coli His/Myc 2-101 aa
Rat S100A4-4872R-B Recombinant Rat S100A4 Protein Pre-coupled Magnetic Beads HEK293
Cattle S100A4-2120C Recombinant Cattle S100A4 protein, His-tagged E.coli His Met1~Lys101 (Accession # P35466)
  • Background
  • Quality Guarantee
  • Case Study
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • Other Resource
S100A4-9.jpg

Fig1. Biological processes associated with S100A4. (Kjetil Boye, 2010)

What is S100A4 protein?

S100A4 (S100 calcium binding protein A4) gene is a protein coding gene which situated on the long arm of chromosome 1 at locus 1q21. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. The S100A4 protein is consisted of 101 amino acids and its molecular mass is approximately 11.7 kDa.

What is the function of S100A4 protein?

S100A4 protein is a secreted protein belonging to the S100 protein family. It is involved in a variety of cellular processes, such as cell growth, differentiation, apoptosis, inflammation and so on. In particular, the S100A4 protein plays an important role in the development and progression of tumors. It can promote the invasion and metastasis of tumor cells, inhibit the apoptosis of tumor cells, and participate in the regulation of tumor microenvironment.

S100A4 Related Signaling Pathway

The S100A4 protein, also known as Metastasin-1 or Mts1, It activates MAPK (mitogen-activated protein kinase), NF-κB (nuclear factor κB) and other signaling pathways by interacting with the Receptor for Advanced Glycation End products (RAGE), and affects a variety of cell functions. In addition, S100A4 affects the function of downstream effectors such as Calmodulin kinase, calmodulin, and calcium/calmodulin dependent protein kinase II (CaMKII) by regulating intracellular calcium ion concentration. It also interacts with actin and other cytoskeleton-associated proteins and is involved in cytoskeletal recombination and cell motility. S100A4 may also affect cell cycle regulation and apoptosis.

S100A4-10.jpg

Fig2. Silencing of S100A4 downregulated the PI3K/Akt/mTOR pathway, enhanced autophagy, and promoted wound healing of rabbit cornea after alkali burn. (Yulin Wang, 2020)

S100A4 Related Diseases

S100A4 plays an important role in tumor invasion and metastasis and is considered to be a marker of malignant transformation and poor prognosis. In addition, S100A4 is also associated with a variety of diseases such as inflammatory diseases, bone diseases, cardiovascular diseases, and neurological diseases, and further affects the development and progression of diseases by participating in biological processes such as cell proliferation, migration, and apoptosis.

Bioapplications of S100A4

S100A4's role in promoting tumor growth and metastasis makes it a potential target for cancer therapy. The development of small molecule inhibitor or antibody drugs targeting S100A4 can help inhibit the proliferation and aggressiveness of cancer cells.

Case study 1: Yana Qi, 2022

Metastasis is the major cause of cancer-related mortalities. A tumor-supportive microenvironment, also known as the premetastatic niche at secondary tumor sites, plays a crucial role in metastasis. Remodeling of the extracellular matrix (ECM) is essential for premetastatic niche formation, especially for circulating tumor cell colonization. However, the underlying molecular mechanism that contributes to this effect remains unclear. Here, the researchers developed a lung metastasis model with 4T1 breast cancer cells and found that the metastasis critically depended on the early recruitment of macrophages to the lung. Disruption of macrophage recruitment reduced fibroblast activation and lung metastasis. Furthermore, they identified the secreted protein S100A4, which is produced by M2 macrophages and participates in fibroblast activation and ECM protein deposition via the ERK signaling pathway. Collectively, these results indicate that recruiting S100A4-expressing inflammatory macrophages plays a vital role in ECM remodeling in the premetastatic niche and may act as a potential therapeutic target for breast cancer lung metastasis.

S100A4-3.jpg

Fig1. L929 cells were treated with rS100A4 at different concentrations, and the protein levels of α-SMA and fibronectin were assessed via western blotting.

S100A4-4.jpg
Fig2. The migration ability of the control and rS100A4-treated groups was evaluated by Transwell assays (∗P < 0.05).

Case study 2: Anfei Liu, 2023

Lung metastasis is the leading cause of breast cancer-related death. The tumor microenvironment contributes to the metastatic colonization of tumor cells in the lungs. Tumor secretory factors are important mediators for the adaptation of cancer cells to foreign microenvironments. Here, the researchers report that tumor-secreted stanniocalcin 1 (STC1) promotes the pulmonary metastasis of breast cancer by enhancing the invasiveness of tumor cells and promoting angiogenesis and lung fibroblast activation in the metastatic microenvironment. The results show that STC1 modifies the metastatic microenvironment through its autocrine action on breast cancer cells. Specifically, STC1 upregulates the expression of S100 calcium-binding protein A4 (S100A4) by facilitating the phosphorylation of EGFR and ERK signaling in breast cancer cells. S100A4 mediates the effect of STC1 on angiogenesis and lung fibroblasts. Importantly, S100A4 knockdown diminishes STC1-induced lung metastasis of breast cancer. Moreover, activated JNK signaling upregulates STC1 expression in breast cancer cells with lung-tropism.

S100A4-5.jpg

Fig3. Western blot analysis and the quantification of S100A4 of MDA-MB-231 cells with or without 1-h pretreatment with 100 ng/mL of rhSTC1.

S100A4-6.jpg
Fig4. Validation of S100A4 knockdown and overexpression in human breast cancer cell lines, MDA-MB-231-STC1 and LM2-shSTC1, by Western blot analyses.
S100A4-7.jpg

Fig1. S100A4 proteins get involved in NF-kB and AKT signaling, affecting the cell activities. (Ting Wang, 2023)

S100A4-8.jpg

Fig2. Schematic diagram depicting the roles of STC1 in breast cancer pulmonary metastasis. (Anfei Liu, 2023)

S100A4 involved in several pathways and played different roles in them. We selected most pathways S100A4 participated on our site, such as , which may be useful for your reference. Also, other proteins which involved in the same pathway with S100A4 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein

S100A4 has several biochemical functions, for example, RAGE receptor binding, actin binding, calcium ion binding. Some of the functions are cooperated with other proteins, some of the functions could acted by S100A4 itself. We selected most functions S100A4 had, and list some proteins which have the same functions with S100A4. You can find most of the proteins on our site.

Function Related Protein
RAGE receptor bindingS100P;HMGB1;S100A8;S100B;HMGB2;S100A13;S100A12;FPR1;S100A9
actin bindingABRAB;TNNT2A;SYNPO2L;KLHL2;PHACTR4B;INO80;VILL;INPPL1A;IPP
calcium ion bindingNKD2A;PLSCR4;SYT17;PLCH2;EFCAB4B;SGCA;PCDH2G10;EEF2K;PCDH1GB9
calcium-dependent protein bindingANXA6;C9orf9;TNNI3;FCN2;CPNE3;CCDC167;TSG101;MBL2;PDCD6IP
identical protein bindingUHRF1;NELL2;TOPBP1;EGFR;ZDHHC17;CNTN2;TLR3;LUC7L;GNPNAT1
poly(A) RNA bindingRBM34;CAST;MSI2;RBM6;PAN3;EIF3H;FUS;KHDRBS1;RPS12
protein bindingNR5A2;PRKG1;PITX1;MIIP;NOP56;EPO;CLK2;SETX;PLEKHA1

S100A4 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with S100A4 here. Most of them are supplied by our site. Hope this information will be useful for your research of S100A4.

TP53; S100B; MDM2; AREGB; BTC

Research Area

Related articles

Grum-Schwensen, B; Klingelhofer, J; et al. S100A4-neutralizing antibody suppresses spontaneous tumor progression, pre-metastatic niche formation and alters T-cell polarization balance. BMC CANCER 15:-(2015).
Wang, DG; Zhang, JW; et al. Functional expression, characterization and application of the human S100A4 protein. MOLECULAR MEDICINE REPORTS 11:175-181(2015).
  • Reviews
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Customer Reviews (3)

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10/07/2022

    I have been using these for several years and they have been very stable and I swear by this product.

    04/14/2022

      The experimental process is simple and there is no special environmental requirement.

      11/20/2021

        It can remain stable under different temperature and pH conditions.

        Q&As (6)

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        What is the current progress in drug development for S100A4? 04/28/2022

        Drug development for S100A4 is currently in clinical trials, including small molecule inhibitors against S100A4 and inhibitors against the S100A4 signaling pathway.

        Is the expression of S100A4 affected by environmental factors? 11/08/2021

        Yes, the expression of S100A4 is affected by environmental factors, such as nutritional status, inflammation level, etc.

        What role does S100A4 play in tumor immunotherapy? 08/10/2021

        S100A4 plays an important role in tumor immunotherapy, it can act as a tumor-associated antigen, activate the immune response of tumor cells, promote apoptosis of tumor cells and inhibit the proliferation of tumor cells.

        How to improve the nutritional status of S100A4 protein through diet? 02/19/2021

        There is no conclusive evidence that diet improves the nutritional status of S100A4 protein, but maintaining a balanced diet and moderate protein intake can help maintain good health.

        How can S100A4 be used as a biomarker in clinical practice? 01/22/2020

        In clinical practice, the expression level of S100A4 can be detected to predict disease progression and prognosis, and to guide the choice of treatment regimen. For example, in the diagnosis and treatment of oncological diseases.

        What is the relationship between S100A4 protein and muscle wasting? 12/31/2019

        Studies have shown that S100A4 protein can inhibit the occurrence of muscle wasting, but the specific mechanism is not clear.

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