Recombinant Human S100A9 cell lysate
Cat.No. : | S100A9-684HCL |
Product Overview : | Human S100A9 / CAGB / p14 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
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Source : | Baculovirus-Insect Cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Tag : | Non |
Gene Name : | S100A9 S100 calcium binding protein A9 [ Homo sapiens ] |
Official Symbol : | S100A9 |
Synonyms : | S100A9; S100 calcium binding protein A9; CAGB, CFAG, S100 calcium binding protein A9 (calgranulin B) , S100 calcium binding protein A9 (calgranulin B); protein S100-A9; 60B8AG; CGLB; LIAG; MAC387; MIF; MRP14; NIF; P14; MRP-14; calgranulin B; calgranulin-B; calprotectin L1H subunit; leukocyte L1 complex heavy chain; migration inhibitory factor-related protein 14; S100 calcium-binding protein A9 (calgranulin B); CAGB; CFAG; L1AG; |
Gene ID : | 6280 |
mRNA Refseq : | NM_002965 |
Protein Refseq : | NP_002956 |
MIM : | 123886 |
UniProt ID : | P06702 |
Chromosome Location : | 1q21 |
Function : | calcium ion binding; protein binding; signal transducer activity; |
Products Types
◆ Recombinant Protein | ||
S100A9-202H | Recombinant Human S100A9 Protein, MYC/DDK-tagged, C13 and N15-labeled | +Inquiry |
S100A9-231H | Recombinant Human S100A9 Protein, MYC/DDK-tagged | +Inquiry |
S100a9-1362H | Recombinant Rat S100a9 Protein, His-SUMO-tagged | +Inquiry |
S100A9-233H | Recombinant Human S100A9 Protein, His-tagged | +Inquiry |
S100A9-3590M | Recombinant Full Length Mouse S100A9, His-tagged | +Inquiry |
◆ Native Protein | ||
S100A9-3179H | Native Human S100A9 protein(Met1-Pro114) | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Customer Reviews (3)
Write a reviewThis includes complementary reagents, protocols, and access to an extensive knowledge base, all aimed at streamlining the trial process and maximizing the chances of achieving meaningful results.
By choosing the S100A9 protein and partnering with the manufacturer, researchers can confidently conduct their trials, achieve reliable results, and contribute to groundbreaking discoveries.
Collaborating with the manufacturer opens up possibilities for potential collaboration and exchange of information with a wider scientific community, allowing researchers to stay at the forefront of advancements in their respective fields.
Q&As (5)
Ask a questionS100A9 levels can be measured using various laboratory techniques, such as ELISA (enzyme-linked immunosorbent assay).
Some research is exploring the development of drugs that target S100A9 to modulate inflammation, but clinical applications are still in the experimental phase.
Yes, elevated S100A9 levels have been associated with conditions like rheumatoid arthritis and inflammatory bowel disease, making it a potential biomarker.
Elevated S100A9 levels have been linked to poorer outcomes in certain diseases, making it a potential predictor of disease severity.
Genetic variations can influence S100A9 expression, and these variations may impact an individual's susceptibility to certain diseases.
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