S100A8

  • Official Full Name

    S100 calcium binding protein A8

  • Overview

    The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. [provided by RefSeq, Jul 2008]
  • Synonyms

    S100A8; S100 calcium binding protein A8; P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG; protein S100-A8; MRP-8; calgranulin A; calgranulin-A; cystic fibrosis antigen; calprotectin L1L subunit; urinary stone protein band A; leukocyte L1 complex light chain; migration inhibitory factor-related protein 8; S100 calcium-binding protein A8 (calgranulin A);

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Protein Pre-coupled Magnetic Beads
  • Cattle
  • Dog
  • Human
  • Mouse
  • Pig
  • Rabbit
  • Rat
  • Rhesus Macaque
  • Baculovirus-Insect Cells
  • E.coli
  • HEK293
  • HEK293F
  • HEK293T
  • Insect Cell
  • Insect Cells
  • Mammalian Cell
  • Mammalian cells
  • Mouse
  • Yeast
  • C
  • His
  • Flag
  • GST
  • His (Fc)
  • Avi
  • His|S
  • His|T7
  • SUMO
  • Myc
  • DDK
  • Myc|DDK
  • N/A
  • N
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human S100A8/S100A9-13H Active Recombinant Human S100A8 (Met1-Glu93) and S100A9 (Thr2-Pro114) Heterodimer Protein E.coli Met1-Glu93 (S100A8) & Thr2-Pro114 (S100A9)
Human S100A8-2216H Active Recombinant Human S100A8, His tagged Insect Cells His Met 1-Glu 93
Human S100A8-3732H Recombinant Human S100A8, His-tagged E.coli His
Human S100A8 & S100A9-2217H Recombinant Human S100A8 & S100A9 Heterodimer Protein, His & Flag tagged Insect Cells His & Flag Met1-Glu93&Met1-Pro114
Human S100A8-2492H Recombinant Human S100A8, GST-tagged E.coli GST 1-93aa
Human S100A8-432H Recombinant Human S100A8 protein E.coli N/A Met 1 - Glu 93
Human S100A8-294H Recombinant Human S100A8 Protein, MYC/DDK-tagged HEK293 N/A
Human S100A8-685HCL Recombinant Human S100A8 cell lysate Insect Cell N/A
Human S100A8-2710H Recombinant Human S100A8 Protein, His-tagged E.coli N-His Met1-Glu93 and Met1-Pro114 co-expression
Human S100A8-226H Recombinant Human S100A8 Protein, MYC/DDK-tagged HEK293 Myc/DDK
Human S100A8-2709H Recombinant Human S100A8 Protein, His-tagged HEK293F N-His Met1-Glu93/Met1-Pro114
Human S100A8-320H Recombinant Human S100A8 protein E.coli 93
Human S100A8-1684HFL Recombinant Full Length Human S100A8 Protein, C-Flag-tagged Mammalian cells Flag
Human S100A8-1013H Active Recombinant Human Calprotectin Protein E.coli His
Human S100A8-2670H Recombinant Human S100A8 Protein, His-tagged E.coli N-His Met1-Glu93
Human S100A8-12H Recombinant Human S100A8 Protein (Leu2-Glu93), N-His-tagged HEK293 N-His Leu2-Glu93
Human S100A8-1943H-B Recombinant Human S100A8 Protein Pre-coupled Magnetic Beads HEK293
Human S100A8-1943H Recombinant Human S100A8 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Human S100A8-2671H Active Recombinant Human S100A8 protein, His-tagged E.coli His Leu2-Glu93
Human S100A8-6219H Recombinant Human S100A8 Protein (Met1-Glu89), C-His tagged E.coli C-His Met1-Glu89
Human S100A8-6220H Recombinant Human S100A8 Protein (Met1-Glu93), C-His tagged E.coli C-His Met1-Glu93
Mouse S100a8-01M Active Recombinant Mouse S100a8 Protein E.coli
Mouse S100a8-5107M Recombinant Mouse S100 Calcium Binding Protein A8 (calgranulin A), His-tagged Mouse His
Mouse S100a8-405M Recombinant Mouse S100A8 protein(Pro2-Glu89), His-tagged E.coli N-His Pro2-Glu89
Mouse S100a8-3545M Recombinant Mouse S100a8, His-tagged E.coli His
Mouse S100A8-14622M Recombinant Mouse S100A8 Protein Mammalian Cell His
Mouse S100a8-7328M Recombinant Mouse S100a8 Protein, His-tagged E.coli His 1-89
Mouse S100a8-129M Recombinant Mouse S100a8 protein (Met1-Glu89), His-tagged Baculovirus-Insect Cells His
Mouse S100a8-5741M Recombinant Mouse S100a8 Protein (Met1-Glu93), C-His tagged E.coli C-His Met1-Glu93
Mouse S100A8-1510M Recombinant Mouse S100A8 Protein (2-89 aa), His-tagged Yeast His 2-89 aa
Mouse S100a8-3463M Recombinant Mouse S100a8 protein, His-SUMO-tagged E.coli His-SUMO 2-89aa
Mouse S100A8-7868M Recombinant Mouse S100A8 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Mouse S100a8-127M Recombinant Mouse S100a8 Protein, His-tagged E.coli His
Mouse S100a8-3464M Recombinant Mouse S100a8 protein, GST-tagged E.coli GST 2-89aa
Mouse S100a8-12HCL Recombinant Mouse S100a8 overexpression lysate HEK293T Myc/DDK
Mouse S100A8-3387M Recombinant Mouse S100A8 protein, His-tagged HEK293 His Met1~Glu89
Mouse S100a8-28HCL Recombinant Mouse S100a8 overexpression lysate HEK293T Myc/DDK
Mouse S100A8-7868M-B Recombinant Mouse S100A8 Protein Pre-coupled Magnetic Beads HEK293
Mouse S100a8-5671M Recombinant Mouse S100a8 Protein, Myc/DDK-tagged HEK293T Myc/DDK
Rat S100A8-5215R Recombinant Rat S100A8 Protein Mammalian Cell His
Rat S100a8-1361R Recombinant Rat S100a8 Protein, His-tagged E.coli His
Rat S100a8-1803R Recombinant Rat S100a8 protein, His & T7-tagged E.coli His/T7 Met1~Glu89 (Accession # P50115)
Rat S100A8-1511R Recombinant Rat S100A8 Protein (2-89 aa), His-tagged Yeast His 2-89 aa
Rat S100a8-2039R Recombinant Rat S100a8 Protein, His-tagged E.coli N-His Met1-Glu89
Rat S100A8-4874R Recombinant Rat S100A8 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Rat S100A8-4874R-B Recombinant Rat S100A8 Protein Pre-coupled Magnetic Beads HEK293
Rabbit S100A8-1804R Recombinant Rabbit S100A8 protein, His & T7-tagged E.coli His/T7 Met1~Ala89 (Accession # G1SXG6)
Dog S100A8-0316D Recombinant Dog S100A8 Protein, His-tagged E.coli His Met1~Glu89
Cattle S100A8-1801C Recombinant Cattle S100A8 protein, His & S-tagged E.coli His/S Met1~Glu89 (Accession # P28782)
Rhesus Macaque S100A8-4064R Recombinant Rhesus monkey S100A8 Protein, His-tagged Mammalian Cell His
Rhesus Macaque S100A8-3881R Recombinant Rhesus Macaque S100A8 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Rhesus Macaque S100A8-3881R-B Recombinant Rhesus Macaque S100A8 Protein Pre-coupled Magnetic Beads HEK293
Pig S100A8-1802P Recombinant Pig S100A8 protein, His & T7-tagged E.coli His/T7 Met1~Glu89 (Accession # C3S7K5)
  • Background
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  • Case Study
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • Other Resource

What is S100A8 protein?

S100A8 (S100 calcium binding protein A8) gene is a protein coding gene which situated on the long arm of chromosome 1 at locus 1q21. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. This protein may function in the inhibition of casein kinase and as a cytokine. The S100A8 protein is consisted of 93 amino acids and its molecular mass is approximately 10.8 kDa.

What is the function of S100A8 protein?

The S100A8 protein is a calcium-binding protein belonging to the S100 family. It has a variety of functions, including regulating cell proliferation, differentiation and apoptosis, participating in inflammatory response and immune regulation, and affecting tumorigenesis and development. Specifically, S100A8 protein can promote the proliferation and migration of fibroblasts, enhance the phagocytosis capacity of macrophages, and inhibit the proliferation and activation of T cells, thus playing an important role in inflammatory response and immune regulation.

S100A8 Related Signaling Pathway

S100A8 protein can bind to receptors, activate signaling pathways such as NF-κB and MAPK, promote the expression and release of inflammatory factors, and thus participate in the occurrence and development of inflammatory response. S100A8 protein can affect the immune response process by regulating the function of immune cells such as T cells, B cells and macrophages. S100A8 protein can participate in the regulation of apoptosis by regulating the permeability of mitochondrial membrane and the activity of caspase family proteins.

S100A8 Related Diseases

In cancer, S100A8 protein is closely related to tumor cell proliferation, metastasis, invasion and other processes, and can be used as tumor markers for diagnosis and treatment monitoring. In inflammatory diseases, such as rheumatoid arthritis and inflammatory bowel disease, the expression level of S100A8 protein is correlated with the severity and prognosis of the disease. In addition, S100A8 protein is also associated with the occurrence and development of cardiovascular diseases such as atherosclerosis and myocardial infarction.

S100A8-9.jpg

Fig1. Leukemia cell apoptosis by S100A8 and S100A9. (Farnaz Razmkhah, 2023)

Bioapplications of S100A8

S100A8 protein is widely used in clinical research for the diagnosis and treatment of diseases. For example, by detecting the expression level of S100A8 protein in patients, it can help diagnose certain autoimmune diseases; At the same time, using the biological characteristics of S100A8 protein, the development of drugs or therapies targeting this protein is expected to provide new ideas and methods for the treatment of related diseases.

Case Study 1: Roseanne Raphael, 2016

Heart failure with ejection fraction (HFpEF) is a syndrome resulting from several co-morbidities in which specific mediators are unknown. The platelet proteome responds to disease processes. The researchers hypothesize that the platelet proteome will change composition in patients with HFpEF and may uncover mediators of the syndrome.

Mass spectrometry identified 6102 proteins with five scans with peptide probabilities of ≥0.85. Of the 6102 proteins, 165 were present only in symptomatic subjects, 78 were only found in outpatient subjects and 157 proteins were unique to the control group. The S100A8 protein was identified consistently in HFpEF samples when compared with controls. They validated the fining that plasma S100A8 levels are increased in subjects with HFpEF (654 ± 391) compared to controls (352 ± 204) in an external cohort (p = 0.002). Recombinant S100A8 had direct effects on the electrophysiological and calcium handling profile in human induced pluripotent stem cell-derived cardiomyocytes.

S100A8-3.jpg

Fig1. Representative MS/MS scan for S100A8 peptide sequence ALNSIIDVYHK.

S100A8-4.jpg
Fig2. Spontaneous Ca2+ transients recorded from human cardiomyocytes treated with rS100A8 as indicated by the blue line.

Case Study 2: Andrew P Coveney, 2015

Myeloid-related protein 8 (Mrp8) is the active component of Mrp8/14 protein complex released by phagocytes at the site of infection and stimulates inflammatory responses. However, it is unclear whether Mrp8 could induce self-tolerance and cross-tolerance to bacterial infection. Here the researchers report that Mrp8 triggered TNF-α and IL-6 release via a Toll-like receptor 4 (TLR4)-dependent manner. Pre-stimulation of murine macrophages and human monocytes with Mrp8 induced self-tolerance to Mrp8 re-stimulation and cross-tolerance to lipopolysaccharide (LPS), bacterial lipoprotein (BLP), gram-negative and gram-positive bacterial challenges, with substantially attenuated TNF-α and IL-6 release. Moreover, Mrp8 tolerisation significantly reduced serum TNF-α and IL-6, increased polymorphonuclear neutrophil (PMN) recruitment and accelerated bacterial clearance, thus protecting mice against LPS-induced lethality and cecal ligation and puncture (CLP)-induced polymicrobial sepsis. In addition to TLR4, TLR2 also contributed to Mrp8-induced inflammatory response and tolerance. Down-regulation of phosphorylated p38 by Mrp8 pre-stimulation was predominantly responsible for the intracellular mechanism of Mrp8-induced tolerance.

S100A8-5.jpg

Fig3. Peritoneal macrophages isolated from C3H/HeN mice were pre-stimulated with increasing doses of mMrp8 for 18 h and re-stimulated with 5 μg/ml mMrp8 for 6 h.

S100A8-6.jpg
Fig4. Mrp8 pre-stimulation attenuates p38 phosphorylation in human monocytes re-stimulated with either Mrp8.
S100A8-7.jpg

Fig1. S100A8/A9 in tumor progression. (Geetha Srikrishna, 2012)

S100A8-8.jpg

Fig2. Interactions between obesity, hyperglycemia, dexamethasone and S100A8/A9 in COVID19. (Nordin M J Hanssen, 2021)

S100A8 involved in several pathways and played different roles in them. We selected most pathways S100A8 participated on our site, such as , which may be useful for your reference. Also, other proteins which involved in the same pathway with S100A8 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein

S100A8 has several biochemical functions, for example, RAGE receptor binding, Toll-like receptor 4 binding, arachidonic acid binding. Some of the functions are cooperated with other proteins, some of the functions could acted by S100A8 itself. We selected most functions S100A8 had, and list some proteins which have the same functions with S100A8. You can find most of the proteins on our site.

Function Related Protein
RAGE receptor bindingHMGB2;S100A8;S100A13;FPR1;S100A9;S100A7;S100A4;S100A12;HMGB1
Toll-like receptor 4 bindingTIRAP;SAA3;S100A9;S100A8
arachidonic acid bindingS100A8;CYP4F14;ALOX5AP;S100A9;PPARG;STX3;CYP4A14
calcium ion bindingMYLZ3;S100T;ANXA3B;EPD;PKD1L2;OCM2;CDHR1;SULF2B;CAMKK2
microtubule bindingZFP207;WDR43;CEP57;POLB;DPYSL2B;GAS2L2;CLASP1;FES;CKAP5
protein bindingSPSB3;BBS9;ZNF143;KAT6B;CTLA4;NGFR;Shh;MYLK2;COG4
zinc ion bindingZNRF2B;KNG1;RABGGTB;BFAR;CXXC1A;CAR1;RNPEP;ZMAT4;ARIH1L

S100A8 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with S100A8 here. Most of them are supplied by our site. Hope this information will be useful for your research of S100A8.

S100A9; GAPDH; ESR1; NOS2; PPP2R2B

Research Area

Related articles

Leal, RF; Planell, N; et al. Identification of inflammatory mediators in patients with Crohn's disease unresponsive to anti-TNF alpha therapy. GUT 64:233-242(2015).
Obry, A; Lequerre, T; et al. Identification of S100A9 as Biomarker of Responsiveness to the Methotrexate/Etanercept Combination in Rheumatoid Arthritis Using a Proteomic Approach. PLOS ONE 9:-(2014).
  • Q&As
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Q&As (7)

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How do alterations in S100A8 expression impact cellular stress responses and aging? 03/03/2022

Changes in S100A8 expression impact cellular stress responses, with implications for aging and age-related diseases.

How does S100A8 contribute to the pathogenesis of cancer, especially in terms of tumor growth and metastasis? 12/12/2021

S100A8 contributes to cancer development, influencing tumor cell proliferation and metastatic potential.

What is the impact of S100A8 on wound healing and tissue repair mechanisms? 03/02/2021

S100A8 is involved in wound healing, affecting tissue repair and regeneration.

How does S100A8 interact with calcium ions and what are the implications for inflammatory processes? 01/18/2021

S100A8 binds calcium ions, modulating inflammatory processes and cytokine production.

How does S100A8 influence the development and progression of cardiovascular diseases? 11/01/2019

S100A8 affects cardiovascular disease development, impacting inflammation and vascular integrity.

What role does S100A8 play in the immune response, particularly in autoimmune diseases? 09/22/2019

S100A8 plays a significant role in the immune response, impacting the pathogenesis of autoimmune diseases.

What are the effects of S100A8 on skin homeostasis and the development of skin disorders? 10/06/2018

S100A8 influences skin health, playing a role in maintaining skin barrier function and in skin disorders.

Customer Reviews (3)

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Reviews
02/01/2022

    Dependable protein quantification, ensures experimental accuracy.

    10/29/2020

      Consistent western blot results, a lab essential.

      03/12/2020

        Expert protein engineering support, essential for our projects.

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