Active Recombinant Full Length Human RPS6KA1 Protein, N-His-tagged

• Cat.No.: RPS6KA1-167HFL
• Product Overview: N-terminal 6His-tagged recombinant full-length human RSK1 expressed in Sf21 insect cells. Purified using Ni2+/NTA agarose. Activated with PDK1 and MAPK2 and repurified using glutathioneSepharose, heparin-Sepharose and Ni2+/NTA agarose.

Specification

• Description: Rsk1/MAPKAP Kinase 1a is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) Signaling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 1.632mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl pH7.5, 0.03% Brij-35, 0.1mM EGTA, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 3145U/mg, where one unit of RSK1 activity is defined as 1nmol phosphate incorporated into 30μM (KKKNRTLSVA) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 84 kDa
• AA Sequence: 1 MHHHHHHEFM PLAQLKEPWP LMELVPLDPE NGQTSGEEAG LQPSKDEGVL KEISITHHVK 61 AGSEKADPSH FELLKVLGQG SFGKVFLVRK VTRPDSGHLY AMKVLKKATL KVRDRVRTKM 121 ERDILADVNH PFVVKLHYAF QTEGKLYLIL DFLRGGDLFT RLSKEVMFTE EDVKFYLAEL 181 ALGLDHLHSL GIIYRDLKPE NILLDEEGHI KLTDFGLSKE AIDHEKKAYS FCGTVEYMAP 241 EVVNRQGHSH SADWWSYGVL MFEMLTGSLP FQGKDRKETM TLILKAKLGM PQFLSTEAQS 301 LLRALFKRNP ANRLGSGPDG AEEIKRHVFY STIDWNKLYR REIKPPFKPA VAQPDDTFYF 361 DTEFTSRTPK DSPGIPPSAG AHQLFRGFSF VATGLMEDDG KPRAPQAPLH SVVQQLHGKN 421 LVFSDGYVVK ETIGVGSYSE CKRCVHKATN MEYAVKVIDK SKRDPSEEIE ILLRYGQHPN 481 IITLKDVYDD GKHVYLVTEL MRGGELLDKI LRQKFFSERE ASFVLHTIGK TVEYLHSQGV 541 VHRDLKPSNI LYVDESGNPE CLRICDFGFA KQLRAENGLL MTPCYTANFV APEVLKRQGY 601 DEGCDIWSLG ILLYTMLAGY TPFANGPSDT PEEILTRIGS GKFTLSGGNW NTVSETAKDL 661 VSKMLHVDPH QRLTAKQVLQ HPWVTQKDKL PQSQLSHQDL QLVKGAMAAT YSALNSSKPT 721 PQLKPIESSI LAQRRVRKLP STTL
• Purity: Purity 94.6% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at - 70 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: RPS6KA1 ribosomal protein S6 kinase A1 [ Homo sapiens (human) ]
• Official Symbol: RPS6KA1
• Synonyms: RPS6KA1; ribosomal protein S6 kinase A1; RSK; HU-1; RSK1; p90Rsk; MAPKAPK1; MAPKAPK1A
• Gene ID: 6195
• mRNA Refseq: NM_002953.4
• Protein Refseq: NP_002944.2
• MIM: 601684
• UniProt ID: Q15418