Recombinant Human RPS6KA1 Protein (Q33-T353), Tag Free

• Cat.No.: RPS6KA1-1148H
• Product Overview: Recombinant Human RSK1(Q33-T353) Protein was expressed in Insect cell.

Specification

• Description: Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the pre-initiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4). Mediates induction of hepatocyte prolifration by TGFA through phosphorylation of CEBPB. Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression. Phosphorylates EPHA2 at 'Ser-897', the RPS6KA-EPHA2 signaling pathway controls cell migration.
• Source: Insect cell
• Species: Human
• Tag: Non
• Form: Liquid
• Protein Length: Q33-T353
• Endotoxin: < 0.01 EU per μg of the protein
• Purity: 90%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol
• Shipping: It is shipped out with blue ice.

Gene Information

• Gene Name: RPS6KA1 ribosomal protein S6 kinase, 90kDa, polypeptide 1 [ Homo sapiens (human) ]
• Official Symbol: RPS6KA1
• Synonyms: RPS6KA1; ribosomal protein S6 kinase, 90kDa, polypeptide 1; ribosomal protein S6 kinase, 90kD, polypeptide 1; ribosomal protein S6 kinase alpha-1; HU 1; RSK; RSK1; RSK-1; p90S6K; p90RSK1; p90-RSK 1; MAPKAPK-1a; S6K-alpha 1; S6K-alpha-1; MAPKAP kinase 1a; ribosomal S6 kinase 1; MAPK-activated protein kinase 1a; ribosomal protein S6 kinase alpha 1; 90 kDa ribosomal protein S6 kinase 1; MAP kinase-activated protein kinase 1a; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1); HU-1; MAPKAPK1A
• Gene ID: 6195
• mRNA Refseq: NM_001006665
• Protein Refseq: NP_001006666
• MIM: 601684
• UniProt ID: Q15418

Reviews

07/10/2022

Exceptional protein purification, highly recommended for research.

06/02/2022

Dependable mass spectrometry analysis; enhances our proteomics research.

05/26/2020

Quick and precise protein expression, a valuable service.

Q&As

What is the impact of RPS6KA1 dysregulation on metabolic disorders, such as diabetes and obesity?

Dysregulation of RPS6KA1 impacts metabolic processes, influencing conditions like diabetes and obesity.

What are the mechanisms by which RPS6KA1 modulates the cell cycle and apoptosis?

RPS6KA1 modulates the cell cycle and apoptosis, playing a role in cell proliferation and programmed cell death.

How does RPS6KA1 influence neuronal development and function, especially in learning and memory processes?

RPS6KA1 is involved in neuronal development and function, affecting processes like learning and memory.

How do genetic mutations in RPS6KA1 contribute to the development of certain cancers and other diseases?

Genetic mutations in RPS6KA1 are linked to certain cancers and diseases, affecting its kinase activity and signaling functions.

How does RPS6KA1 interact with other kinases and signaling molecules in cellular signaling networks?

RPS6KA1 interacts with various kinases and signaling molecules, integrating multiple cellular signaling pathways.

What role does RPS6KA1 play in cellular responses to stress, particularly in cancer cells?

RPS6KA1 plays a crucial role in stress responses in cancer cells, influencing cell survival and adaptation mechanisms.

How does RPS6KA1 regulate protein synthesis and cell growth through the mTOR signaling pathway?

RPS6KA1 regulates protein synthesis and cell growth by modulating the mTOR pathway, affecting ribosomal protein activity.