Recombinant Human RPS6KA1 293 Cell Lysate

• Cat.No.: RPS6KA1-2162HCL

Specification

• Description: Antigen standard for ribosomal protein S6 kinase, 90kDa, polypeptide 1 (RPS6KA1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: RPS6KA1 ribosomal protein S6 kinase, 90kDa, polypeptide 1 [ Homo sapiens ]
• Official Symbol: RPS6KA1
• Synonyms: RPS6KA1; ribosomal protein S6 kinase, 90kDa, polypeptide 1; ribosomal protein S6 kinase, 90kD, polypeptide 1; ribosomal protein S6 kinase alpha-1; HU 1; RSK; RSK1; RSK-1; p90S6K; p90RSK1; p90-RSK 1; MAPKAPK-1a; S6K-alpha 1; S6K-alpha-1; MAPKAP kinase 1a; ribosomal S6 kinase 1; MAPK-activated protein kinase 1a; ribosomal protein S6 kinase alpha 1; 90 kDa ribosomal protein S6 kinase 1; MAP kinase-activated protein kinase 1a; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1); HU-1; MAPKAPK1A
• Gene ID: 6195
• mRNA Refseq: NM_001006665
• Protein Refseq: NP_001006666
• MIM: 601684
• UniProt ID: Q15418
• Chromosome Location: 1p
• Pathway: Activated TLR4 signalling, organism-specific biosystem; Activation of NMDA receptor upon glutamate binding and postsynaptic events, organism-specific biosystem; Axon guidance, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; CREB phosphorylation, organism-specific biosystem; CREB phosphorylation through the activation of Ras, organism-specific biosystem; Cytoplasmic Ribosomal Proteins, organism-specific biosystem
• Function: ATP binding; cysteine-type endopeptidase inhibitor activity involved in apoptotic process; magnesium ion binding; nucleotide binding; protein binding; protein serine/threonine kinase activity

Reviews

07/10/2022

Exceptional protein purification, highly recommended for research.

06/02/2022

Dependable mass spectrometry analysis; enhances our proteomics research.

05/26/2020

Quick and precise protein expression, a valuable service.

Q&As

What is the impact of RPS6KA1 dysregulation on metabolic disorders, such as diabetes and obesity?

Dysregulation of RPS6KA1 impacts metabolic processes, influencing conditions like diabetes and obesity.

What are the mechanisms by which RPS6KA1 modulates the cell cycle and apoptosis?

RPS6KA1 modulates the cell cycle and apoptosis, playing a role in cell proliferation and programmed cell death.

How does RPS6KA1 influence neuronal development and function, especially in learning and memory processes?

RPS6KA1 is involved in neuronal development and function, affecting processes like learning and memory.

How do genetic mutations in RPS6KA1 contribute to the development of certain cancers and other diseases?

Genetic mutations in RPS6KA1 are linked to certain cancers and diseases, affecting its kinase activity and signaling functions.

How does RPS6KA1 interact with other kinases and signaling molecules in cellular signaling networks?

RPS6KA1 interacts with various kinases and signaling molecules, integrating multiple cellular signaling pathways.

What role does RPS6KA1 play in cellular responses to stress, particularly in cancer cells?

RPS6KA1 plays a crucial role in stress responses in cancer cells, influencing cell survival and adaptation mechanisms.

How does RPS6KA1 regulate protein synthesis and cell growth through the mTOR signaling pathway?

RPS6KA1 regulates protein synthesis and cell growth by modulating the mTOR pathway, affecting ribosomal protein activity.