Active Recombinant Full Length Rat RPS6KA1 Protein, N-His,HA-tagged

• Cat.No.: RPS6KA1-166RFL
• Product Overview: Recombinant full-length rat RSK1 (residues 1–end), containing additional N-terminal 6His and HA (YPYDVPDYA) tags. Expressed in baculovirus in Sf21 insect cells, and purified using Ni2+/NTA-agarose. Activated with p42 MAPK and PDK1, and repurified by glutathione-, heparin-, and Ni2+/NTA-agarose chromatographies.

Specification

• Description: Rsk1, also known as Ribosomal Protein S6 Kinase A1, is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) Signaling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
• Source: Sf21
• Species: Rat
• Tag: His,HA
• Protein length: Full Length
• Form: 1.851mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 7170U/mg, where one unit of RSK1, active activity is defined as 1nmol phosphate incorporated into 30μM Rsktide per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 89.9 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF AAATMYPYDV PDYALEMPLA QLKEPWPLME 61 LVPLDPENGQ ASGEEAGLQP SKDEGILKEI SITHHVKAGS EKADPSHFEL LKVLGQGSFG 121 KVFLVRKVTR PDNGHLYAMK VLKKATLKVR DRVRTKMERD ILADVNHPFV VKLHYAFQTE 181 GKLYLILDFL RGGDLFTRLS KEVMFTEEDV KFYLAELALG LDHLHSLGII YRDLKPENIL 241 LDEEGHIKLT DFGLSKEAID HEKKAYSFCG TVEYMAPEVV NRQGHTHSAD WWSYGVLMFE 301 MLTGSLPFQG KDRKETMTLI LKAKLGMPQF LSTEAQSLLR ALFKRNPANR LGSGPDGAEE 361 IKRHIFYSTI DWNKLYRREI KPPFKPAVAQ PDDTFYFDTE FTSRTPRDSP GIPPSAGAHQ 421 LFRGFSFVAT GLMEDDSKPR ATQAPLHSVV QQLHGKNLVF SDGYIVKETI GVGSYSVCKR 481 CVHKATNMEY AVKVIDKSKR DPSEEIEILL RYGQHPNIIT LKDVYDDSKH VYLVTELMRG 541 GELLDKILRQ KFFSEREASF VLYTISKTVE YLHSQGVVHR DLKPSNILYV DESGNPECLR 601 ICDFGFAKQL RAENGLLMTP CYTANFVAPE VLKRQGYDEG CDIWSLGVLL YTMLAGYTPF 661 ANGPSDTPEE ILTRISSGKF TLNGGNWNTV SETAKDLVSK MLHVDPHQRL TAKQVLQHPW 721 ITQKDKLPQS QLSHQDLQLV KGAMAATYSA LSSSKPTPQL KPIESSILAQ RRVRKLPSTT 781 L
• Purity: Purity 87%.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: Rps6ka1 ribosomal protein S6 kinase A1 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Rps6ka1
• Synonyms: Rps6ka1; ribosomal protein S6 kinase A1; S6K-alpha 1
• Gene ID: 81771
• mRNA Refseq: NM_031107.2
• Protein Refseq: NP_112369.2
• UniProt ID: Q63531