Active Recombinant Human RPS6KA4(2-end) Protein, N-His-tagged

• Cat.No.: RPS6KA4-162H
• Product Overview: N-terminal 6Histagged, recombinant, human MSK2, amino acids 2–end, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTAagarose. Activated using MAPK2 and repurified using Ni2+/NTAagarose.

Specification

• Description: This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including CREB1 and ATF1. The encoded protein can also phosphorylate histone H3 to regulate certain inflammatory genes. Several transcript variants encoding different isoforms have been found for this gene.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 2-end
• Form: 0.9285mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol, 0.03% Brij-35, 270mM sucrose. Frozen solution.
• Bio-activity: 130U/mg, where one unit of MSK2, active activity is defined as 1nmol phosphate incorporated into 30μM modified Crosstide (GRPRTSSFAEGKK) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 89.9 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMGIRN SKAYVDGDED DDESCAVELR ITEANLTGHE 61 EKVSVENFEL LKVLGTGAYG KVFLVRKAGG HDAGKLYAMK VLRKAALVQR AKTQEHTRTE 121 RSVLELVRQA PFLVTLHYAF QTDAKLHLIL DYVSGGEMFT HLYQRQYFKE AEVRVYGGEI 181 VLALEHLHKL GIIYRDLKLE NVLLDSEGHI VLTDFGLSKE FLTEEKERTF SFCGTIEYMA 241 PEIIRSKTGH GKAVDWWSLG ILLFELLTGA SPFTLEGERN TQAEVSRRIL KCSPPFPPRI 301 GPVAQDLLQR LLCKDPKKRL GAGPQGAQEV RNHPFFQGLD WVALAARKIP APFRPQIRSE 361 LDVGNFAEEF TRLEPVYSPP GSPPPGDPRI FQGYSFVAPS ILFDHNNAVM TDGLEAPGAG 421 DRPGRAAVAR SAMMQDSPFF QQYELDLREP ALGQGSFSVC RRCRQRQSGQ EFAVKILSRR 481 LEANTQREVA ALRLCQSHPN VVNLHEVHHD QLHTYLVLEL LRGGELLEHI RKKRHFSESE 541 ASQILRSLVS AVSFMHEEAG VVHRDLKPEN ILYADDTPGA PVKIIDFGFA RLRPQSPGVP 601 MQTPCFTLQY AAPELLAQQG YDESCDLWSL GVILYMMLSG QVPFQGASGQ GGQSQAAEIM 661 CKIREGRFSL DGEAWQGVSE EAKELVRGLL TVDPAKRLKL EGLRGSSWLQ DGSARSSPPL 721 RTPDVLESSG PAVRSGLNAT FMAFNRGKRE GFFLKSVENA PLAKRRKQKL RSATASRRGS 781 PAPANPGRAP VASKGAPRRA NGPLPPS
• Purity: Purity 64% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: RPS6KA4 ribosomal protein S6 kinase A4 [ Homo sapiens (human) ]
• Official Symbol: RPS6KA4
• Synonyms: RPS6KA4; ribosomal protein S6 kinase A4; MSK2; RSK-B; S6K-alpha-4
• Gene ID: 8986
• mRNA Refseq: NM_003942.3
• Protein Refseq: NP_003933.1
• MIM: 603606
• UniProt ID: O75676