Recombinant Human RPS6KA4 293 Cell Lysate

• Cat.No.: RPS6KA4-2160HCL

Specification

• Description: Antigen standard for ribosomal protein S6 kinase, 90kDa, polypeptide 4 (RPS6KA4), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: RPS6KA4 ribosomal protein S6 kinase, 90kDa, polypeptide 4 [ Homo sapiens ]
• Official Symbol: RPS6KA4
• Synonyms: RPS6KA4; ribosomal protein S6 kinase, 90kDa, polypeptide 4; ribosomal protein S6 kinase, 90kD, polypeptide 4; ribosomal protein S6 kinase alpha-4; MSK2; RSK B; RSKB; S6K-alpha-4; ribosomal protein kinase B; ribosomal protein S6 kinase alpha 4; 90 kDa ribosomal protein S6 kinase 4; mitogen- and stress-activated protein kinase 2; nuclear mitogen- and stress-activated protein kinase 2; RSK-B
• Gene ID: 8986
• mRNA Refseq: NM_001006944
• Protein Refseq: NP_001006945
• MIM: 603606
• UniProt ID: O75676
• Chromosome Location: 11q11-q13
• Pathway: Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; ErbB1 downstream signaling, organism-specific biosystem; Insulin Signaling, organism-specific biosystem; L1CAM interactions, organism-specific biosystem; MAPK signaling pathway, organism-specific biosystem; MAPK signaling pathway, conserved biosystem
• Function: ATP binding; magnesium ion binding; mitogen-activated protein kinase p38 binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; protein serine/threonine kinase activity; ribosomal protein S6 kinase activity