Active Recombinant Human TNFRSF14 protein, Fc-tagged

• Cat.No.: TNFRSF14-1538H
• Product Overview: Recombinant Human TNFRSF14 protein(Leu39-Val202), fused with Fc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human LIGHT, Mouse IgG2a Fc Tag, low endotoxin at 2 μg/mL (100 μL/well) can bind Human HVEM, Fc Tag with a linear range of 0.3-20 ng/mL.Immobilized Human HVEM, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human BTLA (31-134), Fc,Avitag with a linear range of 0.156-5 μg/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 43.5 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Leu39-Val202
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>95% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: TNFRSF14 tumor necrosis factor receptor superfamily, member 14 [ Homo sapiens ]
• Official Symbol: TNFRSF14
• Synonyms: TNFRSF14; tumor necrosis factor receptor superfamily, member 14; tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator); tumor necrosis factor receptor superfamily member 14; ATAR; CD270; herpesvirus entry mediator; HVEA; HVEM; LIGHTR; TR2; CD40-like protein; herpesvirus entry mediator A; herpes virus entry mediator A; tumor necrosis factor receptor-like 2; tumor necrosis factor receptor-like gene2
• Gene ID: 8764
• mRNA Refseq: NM_003820
• Protein Refseq: NP_003811
• MIM: 602746
• UniProt ID: Q92956