Recombinant Human MED21, His-tagged

• Cat.No.: MED21-7607H
• Product Overview: Recombinant human MED21 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: MED21 is a member of the mediator complex subunit 21 family. The protein interacts with the human RNA polymerase II holoenzyme and is involved in transcriptional regulation of RNA polymerase II transcribed genes. A pseudogene of this gene is located on chromosome 8. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
• Molecular Mass: 18.0 kDa (167aa)
• Protein length: 1-144aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMADRLTQ LQDAVNSLAD QFCNAIGVLQ QCGPPASFNN IQTAINKDQP ANPTEEYAQL FAALIARTAK DIDVLIDSLP SEESTAALQA ASLYKLEEEN HEAATCLEDV VYRGDMLLEK IQSALADIAQ SQLKTRSGTH SQSLPDS
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term. For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml?

Gene Information

• Gene Name: MED21 mediator complex subunit 21 [ Homo sapiens ]
• Official Symbol: MED21
• Synonyms: MED21; mediator complex subunit 21; SRB7 (suppressor of RNA polymerase B, yeast) homolog , SRB7 suppressor of RNA polymerase B homolog (yeast) , SURB7; mediator of RNA polymerase II transcription subunit 21; SRB7; hSrb7; RNAPII complex component SRB7; RNA polymerase II complex component SRB7; RNA polymerase II holoenzyme component SRB7; SRB7 suppressor of RNA polymerase B homolog; SURB7
• Gene ID: 9412
• mRNA Refseq: NM_004264
• Protein Refseq: NP_004255
• MIM: 603800
• UniProt ID: Q13503
• Chromosome Location: 12p12
• Pathway: Developmental Biology, organism-specific biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; PPARA Activates Gene Expression, organism-specific biosystem; Regulation of Lipid Metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha), organism-specific biosystem; Transcriptional Regulation of White Adipocyte Differentiation, organism-specific biosystem
• Function: DNA-directed RNA polymerase activity; transcription coactivator activity