Recombinant Human MED21 protein, His-tagged

• Cat.No.: MED21-3380H
• Product Overview: Recombinant Human MED21 protein(1-144 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 1-144 aa
• AA Sequence: MADRLTQLQDAVNSLADQFCNAIGV LQQCGPPASFNNIQTAINKDQPANP TEEYAQLFAALIARTAKDIDVLIDS LPSEESTAALQAASLYKLEEENHEA ATCLEDVVYRGDMLLEKIQSALADI AQSQLKTRSGTHSQSLPDS
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: MED21 mediator complex subunit 21 [ Homo sapiens ]
• Official Symbol: MED21
• Synonyms: MED21; mediator complex subunit 21; SRB7 (suppressor of RNA polymerase B, yeast) homolog , SRB7 suppressor of RNA polymerase B homolog (yeast) , SURB7; mediator of RNA polymerase II transcription subunit 21; SRB7; hSrb7; RNAPII complex component SRB7; RNA polymerase II complex component SRB7; RNA polymerase II holoenzyme component SRB7; SRB7 suppressor of RNA polymerase B homolog; SURB7
• Gene ID: 9412
• mRNA Refseq: NM_004264
• Protein Refseq: NP_004255
• MIM: 603800
• UniProt ID: Q13503