| Description : |
c-Jun N-terminal kinases (JNKs), a stress-activated protein kinase (SAPK), is involved in cellular responses to environmental stresses. JNK kinases can be activated by stimuli such as UV light, radiation, protein synthesis inhibitors, ceramide, DNA-damaging drugs, chemopreventive drugs, TNF-a, and interleukin 1. JNK binds to an NH2-terminal region of ATF2 and c-Jun and phosphorylates two sites within the activation domain of each transcription factor. Three JNK genes (Jnk1, -2 and -3) have been identified in humans; however, splice variants result in a total of 10 isoforms. Therapeutic inhibition of JNK may provide clinical benefit in disease as diverse as immune inflammatory (joint, bowel, pulmonary), ischemic injury (brain, cardiac) and neurodegenerative (Alzheimer's, Parkinson's) diseases. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
Full Length |
| Form : |
2.039mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
141U/mg, where one unit of JNK1alpha1/SAPK1c activity is defined as 1nmol phosphate incorporated into 3μM ATF-2 (cat# 12-367) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
45 kDa |
| AA Sequence : |
1 MHHHHHHMSR SKRDNNFYSV EIGDSTFTVL KRYQNLKPIG SGAQGIVCAA YDAILERNVA 61 IKKLSRPFQN QTHAKRAYRE LVLMKCVNHK NIIGLLNVFT PQKSLEEFQD VYIVMELMDA 121 NLCQVIQMEL DHERMSYLLY QMLCGIKHLH SAGIIHRDLK PSNIVVKSDC TLKILDFGLA 181 RTAGTSFMMT PYVVTRYYRA PEVILGMGYK ENVDLWSVGC IMGEMVCHKI LFPGRDYIDQ 241 WNKVIEQLGT PCPEFMKKLQ PTVRTYVENR PKYAGYSFEK LFPDVLFPAD SEHNKLKASQ 301 ARDLLSKMLV IDASKRISVD EALQHPYINV WYDPSEAEAP PPKIPDKQLD EREHTIEEWK 361 ELIYKEVMDL EERTKNGVIR GQPSPLAQVQ Q |
| Purity : |
Purity 100% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
1 year at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
