Recombinant Human MAPK8 Protein, His-tagged

• Cat.No.: MAPK8-2420H
• Product Overview: Recombinant Human MAPK8 Protein (Tyr26-Ile321) with a N-His tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 37.6 kDa
  Accurate Molecular Mass: 38 kDa
• Protein length: Tyr26-Ile321
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 80%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: MAPK8 mitogen-activated protein kinase 8 [ Homo sapiens (human) ]
• Official Symbol: MAPK8
• Synonyms: MAPK8; mitogen-activated protein kinase 8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2; mitogen-activated protein kinase 8
  JUN N-terminal kinase
  MAP kinase 8
  c-Jun N-terminal kinase 1
  stress-activated protein kinase 1
  stress-activated protein kinase 1c
  EC 2.7.11.24
• Gene ID: 5599
• mRNA Refseq: NM_139049
• Protein Refseq: NP_620637
• MIM: 601158
• UniProt ID: P45983

Reviews

04/18/2020

I am confident that the MAPK8 protein will unravel crucial insights into molecular mechanisms, greatly enhancing the significance and impact of my scientific investigations.

12/02/2016

By relying on the MAPK8 protein, I can approach my research with unwavering confidence in its quality and the unparalleled support extended by its manufacturer.

02/25/2016

the manufacturer's commitment to providing excellent technical support adds an additional layer of confidence to my choice of the MAPK8 protein.

Q&As

Is MAPK8 involved in inflammatory processes?

Yes, MAPK8 is implicated in regulating inflammation and immune responses, contributing to the pathogenesis of inflammatory diseases like rheumatoid arthritis and inflammatory bowel disease.

Can MAPK8 inhibitors potentially be used in treating Alzheimer's disease?

Research suggests that MAPK8 inhibitors might have therapeutic potential in managing Alzheimer's disease by modulating neuronal responses and reducing neuroinflammation.

How is MAPK8 protein expression assessed in clinical research?

MAPK8 protein expression is often assessed using techniques like immunohistochemistry, western blotting, or quantitative PCR in clinical samples to understand its role in diseases.

How does MAPK8 influence neuronal function and neurodegeneration?

MAPK8 activation is associated with neuronal stress responses and plays a role in neurodegenerative conditions such as Alzheimer's and Parkinson's diseases.

How does MAPK8 influence cellular apoptosis?

MAPK8 signaling pathways can either promote or suppress apoptosis depending on the cellular context and stimuli, making it a potential target for regulating cell death in disease settings.