Recombinant Human MAPK8 Protein, His-tagged

• Cat.No.: MAPK8-2420H
• Product Overview: Recombinant Human MAPK8 Protein (Tyr26-Ile321) with a N-His tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 37.6 kDa
  Accurate Molecular Mass: 38 kDa
• Protein length: Tyr26-Ile321
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 80%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: MAPK8 mitogen-activated protein kinase 8 [ Homo sapiens (human) ]
• Official Symbol: MAPK8
• Synonyms: MAPK8; mitogen-activated protein kinase 8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2; mitogen-activated protein kinase 8
  JUN N-terminal kinase
  MAP kinase 8
  c-Jun N-terminal kinase 1
  stress-activated protein kinase 1
  stress-activated protein kinase 1c
  EC 2.7.11.24
• Gene ID: 5599
• mRNA Refseq: NM_139049
• Protein Refseq: NP_620637
• MIM: 601158
• UniProt ID: P45983