Recombinant Human MAPK8 293 Cell Lysate

Cat.No. : MAPK8-4488HCL
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Description : Antigen standard for mitogen-activated protein kinase 8 (MAPK8), transcript variant JNK1-b2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : MAPK8 mitogen-activated protein kinase 8 [ Homo sapiens ]
Official Symbol : MAPK8
Synonyms : MAPK8; mitogen-activated protein kinase 8; PRKM8; JNK; JNK1; JUN N terminal kinase; SAPK1; MAP kinase 8; JUN N-terminal kinase; c-Jun N-terminal kinase 1; stress-activated protein kinase 1; stress-activated protein kinase 1c; mitogen-activated protein kinase 8 isoform JNK1 beta2; mitogen-activated protein kinase 8 isoform JNK1 alpha1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2;
Gene ID : 5599
mRNA Refseq : NM_139047
Protein Refseq : NP_620635
MIM : 601158
UniProt ID : P45983
Chromosome Location : 10q11
Pathway : ATF-2 transcription factor network, organism-specific biosystem; Activated TLR4 signalling, organism-specific biosystem; Activation of BH3-only proteins, organism-specific biosystem; Activation of BIM and translocation to mitochondria, organism-specific biosystem; Activation of BMF and translocation to mitochondria, organism-specific biosystem; Activation of the AP-1 family of transcription factors, organism-specific biosystem; Adipocytokine signaling pathway, organism-specific biosystem;
Function : ATP binding; JUN kinase activity; MAP kinase activity; histone deacetylase binding; histone deacetylase regulator activity; nucleotide binding; protein binding; protein serine/threonine kinase activity; protein serine/threonine kinase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Is MAPK8 involved in inflammatory processes? 02/23/2023

Yes, MAPK8 is implicated in regulating inflammation and immune responses, contributing to the pathogenesis of inflammatory diseases like rheumatoid arthritis and inflammatory bowel disease.

Can MAPK8 inhibitors potentially be used in treating Alzheimer's disease? 05/18/2022

Research suggests that MAPK8 inhibitors might have therapeutic potential in managing Alzheimer's disease by modulating neuronal responses and reducing neuroinflammation.

How is MAPK8 protein expression assessed in clinical research? 07/21/2020

MAPK8 protein expression is often assessed using techniques like immunohistochemistry, western blotting, or quantitative PCR in clinical samples to understand its role in diseases.

How does MAPK8 influence neuronal function and neurodegeneration? 08/01/2019

MAPK8 activation is associated with neuronal stress responses and plays a role in neurodegenerative conditions such as Alzheimer's and Parkinson's diseases.

How does MAPK8 influence cellular apoptosis? 06/09/2016

MAPK8 signaling pathways can either promote or suppress apoptosis depending on the cellular context and stimuli, making it a potential target for regulating cell death in disease settings.

Customer Reviews (3)

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Reviews
04/18/2020

    I am confident that the MAPK8 protein will unravel crucial insights into molecular mechanisms, greatly enhancing the significance and impact of my scientific investigations.

    12/02/2016

      By relying on the MAPK8 protein, I can approach my research with unwavering confidence in its quality and the unparalleled support extended by its manufacturer.

      02/25/2016

        the manufacturer's commitment to providing excellent technical support adds an additional layer of confidence to my choice of the MAPK8 protein.

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