Recombinant Human MAPK8 Protein (S2-E364), His tagged

• Cat.No.: MAPK8-1137H
• Product Overview: Recombinant Human 6His-TEV-JNK1(S2-E364) (L208I, V219I, C220K, H221G, K222G, I223V, R228T, Y230H) Protein was expressed in E. coli.

Specification

• Description: Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock. Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity. Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteosomal degradation. Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1. In neurons, phosphorylates SYT4 which captures neuronal dense core vesicles at synapses. Phosphorylates EIF4ENIF1/4-ET in response to oxidative stress, promoting P-body assembly. Phosphorylates SIRT6 in response to oxidative stress, stimulating its mono-ADP-ribosyltransferase activity.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid
• Protein length: S2-E364
• Endotoxin: < 0.01 EU per μg of the protein
• Purity: 90%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol
• Shipping: It is shipped out with blue ice.

Gene Information

• Gene Name: MAPK8 mitogen-activated protein kinase 8 [ Homo sapiens (human) ]
• Official Symbol: MAPK8
• Synonyms: MAPK8; mitogen-activated protein kinase 8; PRKM8; JNK; JNK1; JUN N terminal kinase; SAPK1; MAP kinase 8; JUN N-terminal kinase; c-Jun N-terminal kinase 1; stress-activated protein kinase 1; stress-activated protein kinase 1c; mitogen-activated protein kinase 8 isoform JNK1 beta2; mitogen-activated protein kinase 8 isoform JNK1 alpha1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
• Gene ID: 5599
• mRNA Refseq: NM_002750
• Protein Refseq: NP_002741
• MIM: 601158
• UniProt ID: P45983

Reviews

04/18/2020

I am confident that the MAPK8 protein will unravel crucial insights into molecular mechanisms, greatly enhancing the significance and impact of my scientific investigations.

12/02/2016

By relying on the MAPK8 protein, I can approach my research with unwavering confidence in its quality and the unparalleled support extended by its manufacturer.

02/25/2016

the manufacturer's commitment to providing excellent technical support adds an additional layer of confidence to my choice of the MAPK8 protein.

Q&As

Is MAPK8 involved in inflammatory processes?

Yes, MAPK8 is implicated in regulating inflammation and immune responses, contributing to the pathogenesis of inflammatory diseases like rheumatoid arthritis and inflammatory bowel disease.

Can MAPK8 inhibitors potentially be used in treating Alzheimer's disease?

Research suggests that MAPK8 inhibitors might have therapeutic potential in managing Alzheimer's disease by modulating neuronal responses and reducing neuroinflammation.

How is MAPK8 protein expression assessed in clinical research?

MAPK8 protein expression is often assessed using techniques like immunohistochemistry, western blotting, or quantitative PCR in clinical samples to understand its role in diseases.

How does MAPK8 influence neuronal function and neurodegeneration?

MAPK8 activation is associated with neuronal stress responses and plays a role in neurodegenerative conditions such as Alzheimer's and Parkinson's diseases.

How does MAPK8 influence cellular apoptosis?

MAPK8 signaling pathways can either promote or suppress apoptosis depending on the cellular context and stimuli, making it a potential target for regulating cell death in disease settings.