Active Recombinant Human TNFRSF9 Protein, Fc & Avi-tagged, Biotinylated

• Cat.No.: TNFRSF9-346H
• Product Overview: Recombinant Human TNFRSF9 Protein, Fc & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Leu 24 - Gln 186 (Accession # NP_001552.2). Predicted N-terminus: Leu 24. This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag. The protein has a calculated MW of 45.7 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc & Avi
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Human TNFSF9 Ligand, Fc-tag at 2 μg/mL (100 μL/well) can bind Recombinant Human TNFRSF9 protein, Fc & Avi-tagged, Biotinylated with a linear range of 2-19 ng/mL (QC tested).
• Molecular Mass: 45.7 kDa
• Protein length: Leu 24 - Gln 186
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: TNFRSF9 tumor necrosis factor receptor superfamily, member 9 [ Homo sapiens ]
• Official Symbol: TNFRSF9
• Synonyms: TNFRSF9; ILA; 4 1BB; CD137; CD137 antigen; 4-1BB; CDw137; MGC2172; FLJ43501
• Gene ID: 3604
• mRNA Refseq: NM_001561
• Protein Refseq: NP_001552
• MIM: 602250
• UniProt ID: Q07011