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Recombinant Human TNFRSF9 cell lysate

Cat.No. : TNFRSF9-2162HCL
Product Overview : Human 4-1BB / CD137 / TNFRSF9 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : TNFRSF9 tumor necrosis factor receptor superfamily, member 9 [ Homo sapiens ]
Official Symbol : TNFRSF9
Synonyms : TNFRSF9; tumor necrosis factor receptor superfamily, member 9; ILA; tumor necrosis factor receptor superfamily member 9; 4 1BB; CD137; CD137 antigen; T cell antigen ILA; T-cell antigen ILA; 4-1BB ligand receptor; homolog of mouse 4-1BB; receptor protein 4-1BB; T-cell antigen 4-1BB homolog; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63; 4-1BB; CDw137; MGC2172; FLJ43501;
Gene ID : 3604
mRNA Refseq : NM_001561
Protein Refseq : NP_001552
MIM : 602250
UniProt ID : Q07011
Chromosome Location : 1p36
Pathway : Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Downstream signaling in naive CD8+ T cells, organism-specific biosystem;
Function : binding; receptor activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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What diseases are associated with abnormal expression levels of TNFRSF9? 12/10/2022

Studies have found that abnormal expression levels of TNFRSF9 may be associated with certain tumors, autoimmune diseases, and inflammatory diseases.

What is the association of TNFRSF9 with other cytokines? 10/27/2022

It has a variety of associations with other proteins and cytokines. For example, it may interact with the NF-kB signaling pathway to regulate the inflammatory response, and it may also interact with apoptosis-related proteins to regulate the apoptosis process.

What are the main functions of TNFRSF9 in the human body? 07/11/2022

TNFRSF9 mainly plays an important role in the immune system, as an activating signaling molecule, it is able to stimulate the activation, proliferation and survival of T cells and NK cells.

Are there potential therapeutic applications for TNFRSF9 inhibitors or activators? 08/21/2021

Inhibitors or activators targeting TNFRSF9 may have potential applications for the treatment of certain tumors, autoimmune diseases, and inflammatory diseases.

How does TNFRSF9 bind to its ligands? 06/08/2021

The extracellular domain of TNFRSF9 can bind to its ligand, such as 4-1BBL, to initiate signal transduction, which in turn activates downstream signaling pathways.

How is TNFRSF9 related to immuno-oncology? 05/27/2020

TNFRSF9 has some potential in tumor immunotherapy. Several antibody drugs have been developed to use the stimulating effects of TNFRSF9 to enhance the activity of immune cells to fight tumors.

Customer Reviews (3)

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Reviews
03/11/2022

    Good heat resistance, long time treatment at high temperature, still has activity.

    10/11/2021

      The results obtained by SDS-PAGE or Western blot analysis were good.

      07/30/2021

        The purity is good, and the experimental results obtained are accurate.

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