Recombinant Human C4B protein, His/T7-tagged

• Cat.No.: C4B-254H
• Product Overview: Recombinant Human C4B(Asn680~Arg756) fused with His/T7 tag at N-terminal was expressed in E. coli.

Specification

• Description: This gene encodes the basic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. In addition, this gene exists as a long form and a short form due to the presence or absence of a 6.4 kb endogenous HERV-K retrovirus in intron 9.
• Source: E. coli
• Species: Human
• Tag: His/T7
• Form: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Molecular Mass: 12.6kDa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- N VNFQKAINEK LGQYASPTAK RCCQDGVTRL PMMRSCEQRA ARVQQPDCRE PFLSCCQFAE SLRKKSRDKG QAGLQR
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: C4B complement component 4B (Chido blood group) [ Homo sapiens ]
• Official Symbol: C4B
• Synonyms: CH; C4F; CO4; C4B1; C4B2; C4B3; C4B5; C4BD; C4B12; C4B_2; CPAMD3; complement C4-B; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3; Chido form of C4; basic complement C4; complement C4B1a
• Gene ID: 721
• mRNA Refseq: NM_001002029
• Protein Refseq: NP_001002029
• MIM: 120820
• UniProt ID: P0C0L4
• Chromosome Location: 6p21.3
• Pathway: Activation of C3 and C5, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Pertussis, conserved biosystem
• Function: carbohydrate binding; complement binding; endopeptidase inhibitor activity