| Description : |
Protein kinase RNA-regulated (PKR), an ubiquitous serine/threonine kinase that is induced by interferons, is a critical signal transducer implicated in different stress-induced signaling pathways including dsRNA-induced TLR3 signaling and LPS-induced TLR4 signaling. Upon stress stimulation, PKR is activated by autophosphorylation and subsequently phosphorylates the alpha-subunit of eukaryotic initiation factor 2 (eIF-2a) thereby inducing apoptosis. PKR mediates the activation of important transcription factors, such as NF-kB, and MAP kinases, such as p382, by interacting with the TAK1 complex. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
GST,His |
| Protein length : |
252-end |
| Form : |
0.09mg/ml of enzyme in 40mM Tris/HCl pH7.5, 120mM NaCl, 240mM imidazole, 0.08mM EGTA, 20% (v/v) glycerol, 0.8mM benzamidine, 0.16mM PMSF, 0.08% 2- mercaptoethanol. Frozen solution. |
| Bio-activity : |
1189U/mg, where one unit of PKR, active activity is defined as 1nmol phosphate incorporated into 30μM (RSRSRSRSRSRSRSR) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
64 kDa |
| AA Sequence : |
1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSHNQT SLYKKAGTMK 241 ETKYTVDKRF GMDFKEIELI GSGGFGQVFK AKHRIDGKTY VIKRVKYNNE KAEREVKALA 301 KLDHVNIVHY NGCWDGFDYD PETSDDSLES SDYDPENSKN SSRSKTKCLF IQMEFCDKGT 361 LEQWIEKRRG EKLDKVLALE LFEQITKGVD YIHSKKLIHR DLKPSNIFLV DTKQVKIGDF 421 GLVTSLKNDG KRTRSKGTLR YMSPEQISSQ DYGKEVDLYA LGLILAELLH VCDTAFETSK 481 FFTDLRDGII SDIFDKKEKT LLQKLLSKKP EDRPNTSEIL RTLTVWKKSP EKNERHTCGP 541 GPGHHHHHHH HHH |
| Purity : |
Purity 85% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
1 year at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
