| Description : |
HRI (Heme-Regulated eIF2α Kinase) is a serine/threonine protein kinase that is involved in the regulation of protein synthesis in response to changes in heme levels in the cell. HRI is activated in response to decreased heme levels, which can occur during conditions such as iron deficiency, and it phosphorylates the alpha subunit of eukaryotic initiation factor 2 (eIF2α), resulting in a decrease in global protein synthesis and increased translation of mRNAs that contain upstream open reading frames (uORFs) in their 5' untranslated regions. Research has suggested that HRI may also play a role in the response to cellular stress, such as oxidative stress or viral infection. Additionally, HRI has been suggested as a potential therapeutic target in diseases such as sickle cell anemia, where the regulation of hemoglobin synthesis is disrupted, and in viral infections, where the kinase may be involved in the host response to infection. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
GST |
| Protein length : |
140-end |
| Form : |
0.55 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2- mercaptoethanol. Frozen solution. |
| Bio-activity : |
61 U/mg, where one unit of HRI activity is defined as 1 nmol phosphate incorporated into 300 μM RSRSRSRSRSRSRSR per minute at 30 centigrade with a final ATP concentration of 100 μM. |
| Molecular Mass : |
83 kDa |
| AA Sequence : |
1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPEF CEDISRIQKI 241 RSREVALEAQ TSRYLNEFEE LAILGKGGYG RVYKVRNKLD GQYYAIKKIL IKGATKTVCM 301 KVLREVKVLA GLQHPNIVGY HTAWIEHVHV IQPRADRAAI ELPSLEVLSD QEEDREQCGV 361 KNDESSSSSI IFAEPTPEKE KRFGESDTEN QNNKSVKYTT NLVIRESGEL ESTLELQENG 421 LAGLSASSIV EQQLPLRRNS HLEESFTSTE ESSEENVNFL GQTEAQYHLM LHIQMQLCEL 481 SLWDWIVERN KRGREYVDES ACPYVMANVA TKIFQELVEG VFYIHNMGIV HRDLKPRNIF 541 LHGPDQQVKI GDFGLACTDI LQKNTDWTNR NGKRTPTHTS RVGTCLYASP EQLEGSEYDA 601 KSDMYSLGVV LLELFQPFGT EMERAEVLTG LRTGQLPESL RKRCPVQAKY IQHLTRRNSS 661 QRPSAIQLLQ SELFQNSGNV NLTLQMKIIE QEKEIAELKK QLNLLSQDKG VRDDGKDGGV 721 G |
| Purity : |
Purity 85% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
1 year at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
