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Recombinant Human EIF3I 293 Cell Lysate

Cat.No. : EIF3I-6658HCL
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  • Gene Information
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Description : Antigen standard for eukaryotic translation initiation factor 3, subunit I (EIF3I) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : EIF3I eukaryotic translation initiation factor 3, subunit I [ Homo sapiens ]
Official Symbol : EIF3I
Synonyms : EIF3I; eukaryotic translation initiation factor 3, subunit I; EIF3S2, eukaryotic translation initiation factor 3, subunit 2 beta, 36kDa; eukaryotic translation initiation factor 3 subunit I; eIF3 beta; eIF3 p36; eIF3i; TRIP 1; eIF-3-beta; predicted protein of HQ2242; TGFbeta receptor-interacting protein 1; TGF-beta receptor-interacting protein 1; eukaryotic translation initiation factor 3 subunit 2; eukaryotic translation initiation factor 3, subunit 2 beta, 36kDa; eukaryotic translation initiation factor 3, subunit 2 (beta, 36kD); TRIP1; EIF3S2; TRIP-1; PRO2242; eIF3-p36; eIF3-beta;
Gene ID : 8668
mRNA Refseq : NM_003757
Protein Refseq : NP_003748
MIM : 603911
UniProt ID : Q13347
Chromosome Location : 1p34.1
Pathway : Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S, organism-specific biosystem; Cap-dependent Translation Initiation, organism-specific biosystem; Eukaryotic Translation Initiation, organism-specific biosystem; Formation of a pool of free 40S subunits, organism-specific biosystem; Formation of the ternary complex, and subsequently, the 43S complex, organism-specific biosystem; GTP hydrolysis and joining of the 60S ribosomal subunit, organism-specific biosystem; Gene Expression, organism-specific biosystem;
Function : protein binding; contributes_to translation initiation factor activity; translation initiation factor activity; translation initiation factor activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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What is the mechanism of EIF3I protein in protein translation? 06/19/2021

The protein is part of the EIF3 complex, which is involved in mRNA recognition and ligation by interacting with other EIF3 subunits, facilitating the post-transcriptional protein translation process.

Is EIF3I protein involved in cell cycle regulation? 06/02/2021

EIF3I protein may be associated with cell cycle regulation. Some studies have shown that the expression level of EIF3I protein may vary at different stages of the cell cycle, but the specific regulatory mechanism needs further study.

Is there an interaction between the EIF3I protein and other EIF3 subunits? 01/06/2021

Yes, the EIF3I protein can interact with other EIF3 subunits to form the EIF3 complex. The interaction between these subunits is to synergistically regulate the progress of protein synthesis.

Is there a difference in the expression level of EIF3I protein in different tissues? 08/30/2020

Yes, there are differences in the expression levels of EIF3I protein in different tissues. For example, in some tumor tissues, the expression level of the EIF3I protein is significantly elevated.

What research evidence is available for the role of EIF3I protein in disease development? 08/02/2020

EIF3I protein is associated with the occurrence and progression of a variety of diseases. For example, in some tumor cells, elevated levels of EIF3I protein are associated with enhanced proliferation, invasion, and metastasis of tumor cells. In addition, the EIF3I protein has been linked to some neurological diseases, such as Parkinson's disease.

Are there drugs that regulate the expression or function of EIF3I protein? 03/24/2020

There are currently no specific drugs designed to regulate the expression or function of the EIF3I protein. However, drug discovery targeting the EIF3 complex may affect the function of the EIF3I protein.

Customer Reviews (3)

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Reviews
12/09/2020

    Stability of EIF3I is very good, and it can maintain a stable performance even after multiple uses, which gives me more confidence in the experimental results.

    10/24/2020

      The band positions of EIF3I in Western blot were accurate and showed good specificity.

      10/06/2020

        Their service attitude is very good, always patiently answer our questions.

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