Recombinant Human EIF3I 293 Cell Lysate
Cat.No. : | EIF3I-6658HCL |
- Specification
- Gene Information
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Description : | Antigen standard for eukaryotic translation initiation factor 3, subunit I (EIF3I) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | EIF3I eukaryotic translation initiation factor 3, subunit I [ Homo sapiens ] |
Official Symbol : | EIF3I |
Synonyms : | EIF3I; eukaryotic translation initiation factor 3, subunit I; EIF3S2, eukaryotic translation initiation factor 3, subunit 2 beta, 36kDa; eukaryotic translation initiation factor 3 subunit I; eIF3 beta; eIF3 p36; eIF3i; TRIP 1; eIF-3-beta; predicted protein of HQ2242; TGFbeta receptor-interacting protein 1; TGF-beta receptor-interacting protein 1; eukaryotic translation initiation factor 3 subunit 2; eukaryotic translation initiation factor 3, subunit 2 beta, 36kDa; eukaryotic translation initiation factor 3, subunit 2 (beta, 36kD); TRIP1; EIF3S2; TRIP-1; PRO2242; eIF3-p36; eIF3-beta; |
Gene ID : | 8668 |
mRNA Refseq : | NM_003757 |
Protein Refseq : | NP_003748 |
MIM : | 603911 |
UniProt ID : | Q13347 |
Chromosome Location : | 1p34.1 |
Pathway : | Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S, organism-specific biosystem; Cap-dependent Translation Initiation, organism-specific biosystem; Eukaryotic Translation Initiation, organism-specific biosystem; Formation of a pool of free 40S subunits, organism-specific biosystem; Formation of the ternary complex, and subsequently, the 43S complex, organism-specific biosystem; GTP hydrolysis and joining of the 60S ribosomal subunit, organism-specific biosystem; Gene Expression, organism-specific biosystem; |
Function : | protein binding; contributes_to translation initiation factor activity; translation initiation factor activity; translation initiation factor activity; |
Products Types
◆ Recombinant Protein | ||
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EIF3I-1719R | Recombinant Rat EIF3I Protein, His (Fc)-Avi-tagged | +Inquiry |
Eif3i-2781M | Recombinant Mouse Eif3i Protein, Myc/DDK-tagged | +Inquiry |
EIF3I-223H | Recombinant Human EIF3I protein, T7-tagged | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionThe protein is part of the EIF3 complex, which is involved in mRNA recognition and ligation by interacting with other EIF3 subunits, facilitating the post-transcriptional protein translation process.
EIF3I protein may be associated with cell cycle regulation. Some studies have shown that the expression level of EIF3I protein may vary at different stages of the cell cycle, but the specific regulatory mechanism needs further study.
Yes, the EIF3I protein can interact with other EIF3 subunits to form the EIF3 complex. The interaction between these subunits is to synergistically regulate the progress of protein synthesis.
Yes, there are differences in the expression levels of EIF3I protein in different tissues. For example, in some tumor tissues, the expression level of the EIF3I protein is significantly elevated.
EIF3I protein is associated with the occurrence and progression of a variety of diseases. For example, in some tumor cells, elevated levels of EIF3I protein are associated with enhanced proliferation, invasion, and metastasis of tumor cells. In addition, the EIF3I protein has been linked to some neurological diseases, such as Parkinson's disease.
There are currently no specific drugs designed to regulate the expression or function of the EIF3I protein. However, drug discovery targeting the EIF3 complex may affect the function of the EIF3I protein.
Customer Reviews (3)
Write a reviewStability of EIF3I is very good, and it can maintain a stable performance even after multiple uses, which gives me more confidence in the experimental results.
The band positions of EIF3I in Western blot were accurate and showed good specificity.
Their service attitude is very good, always patiently answer our questions.
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